TY - JOUR
T1 - Antibiotic effects against periodontal bacteria in organ cultured tissue
AU - Takeshita, Masaaki
AU - Haraguchi, Akira
AU - Miura, Mayumi
AU - Hamachi, Takafumi
AU - Fukuda, Takao
AU - Sanui, Terukazu
AU - Takano, Aiko
AU - Nishimura, Fusanori
N1 - Funding Information:
This work was supported by Grant‐in‐Aid from Japan Society for the Promotion of Science (no. 15K20623, 25870505). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
Publisher Copyright:
© 2016 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Mechanical reduction of infectious bacteria by using physical instruments is considered the principal therapeutic strategy for periodontal disease; addition of antibiotics is adjunctive. However, local antibiotic treatment, combined with conventional mechanical debridement, has recently been shown to be more effective in periodontitis subjects with type 2 diabetes. This suggests that some bacteria may invade the inflamed inner gingival epithelium, and mechanical debridement alone will be unable to reduce these bacteria completely. Therefore, we tried to establish infected organ culture models that mimic the inner gingival epithelium and aimed to see the effects of antibiotics in these established models. Mouse dorsal skin epithelia were isolated, and periodontal bacteria were injected into the epithelia. Infected epithelia were incubated with test antibiotics, and colony-forming ability was evaluated. Results indicated that effective antibiotics differed according to injected bacteria and the bacterial combinations tested. Overall, in organ culture model, the combination of amoxicillin or cefdinir and metronidazole compensate for the effects of less effective bacterial combinations on each other. This in vitro study would suggest effective periodontal treatment regimens, especially for severe periodontitis.
AB - Mechanical reduction of infectious bacteria by using physical instruments is considered the principal therapeutic strategy for periodontal disease; addition of antibiotics is adjunctive. However, local antibiotic treatment, combined with conventional mechanical debridement, has recently been shown to be more effective in periodontitis subjects with type 2 diabetes. This suggests that some bacteria may invade the inflamed inner gingival epithelium, and mechanical debridement alone will be unable to reduce these bacteria completely. Therefore, we tried to establish infected organ culture models that mimic the inner gingival epithelium and aimed to see the effects of antibiotics in these established models. Mouse dorsal skin epithelia were isolated, and periodontal bacteria were injected into the epithelia. Infected epithelia were incubated with test antibiotics, and colony-forming ability was evaluated. Results indicated that effective antibiotics differed according to injected bacteria and the bacterial combinations tested. Overall, in organ culture model, the combination of amoxicillin or cefdinir and metronidazole compensate for the effects of less effective bacterial combinations on each other. This in vitro study would suggest effective periodontal treatment regimens, especially for severe periodontitis.
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U2 - 10.1002/cre2.48
DO - 10.1002/cre2.48
M3 - Article
AN - SCOPUS:85038009887
VL - 3
SP - 5
EP - 12
JO - Clinical and Experimental Dental Research
JF - Clinical and Experimental Dental Research
SN - 2057-4347
IS - 1
ER -