Antibiotic susceptibility of glutaraldehyde-tolerant Mycobacterium chelonae from bronchoscope washing machines

Kazuyo Nomura, Midori Ogawa, Hiroshi Miyamoto, Tetsuro Muratani, Hatsumi Taniguchi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background Contamination of bronchoalveolar lavage fluid is a major problem in the world. Although 2% glutaraldehyde (GA) is widely used as a disinfectant for bronchoscope cleaning, recently, GA-tolerant mycobacteria have been isolated, which makes this problem more complicated. Methods We studied the susceptibility to GA and antibiotics of mycobacteria isolated from bronchoscope washing machines in our hospital. We also studied the minimum inhibitory concentrations of GA and antibiotics with pump inhibitors. Results Twenty-nine mycobacteria were isolated, of which 26 were Mycobacterium chelonae. Among 18 isolates of M chelonae, excluding 8 isolates in which some results were not reproducible, 50% (9 of 18) were 2% GA-tolerant. One hundred percent (9 of 9) of the GA-tolerant isolates and 11% (1 of 9) of the GA-sensitive isolates were either resistant or intermediately resistant to 2 or 3 classes of antibiotics. Efflux pump inhibitors did not influence the susceptibility to GA and antibiotics. Conclusions It was suggested that there might be an association of GA tolerance with antibiotic resistance in M chelonae. There may a different mechanism(s) other than that involving efflux pumps with regard to GA tolerance and antibiotic resistance in M chelonae. When bronchoscopy-related mycobacterial infections are suspected, physicians and clinical microbiologists should exercise care in handling GA-tolerant mycobacteria, which may be resistant to multiple antibiotics.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalAmerican Journal of Infection Control
Volume32
Issue number4
DOIs
Publication statusPublished - Jun 1 2004
Externally publishedYes

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Mycobacterium chelonae
Bronchoscopes
Glutaral
Anti-Bacterial Agents
Mycobacterium
Microbial Drug Resistance
Disinfectants
Bronchoalveolar Lavage Fluid
Microbial Sensitivity Tests
Bronchoscopy

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Health Policy
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Antibiotic susceptibility of glutaraldehyde-tolerant Mycobacterium chelonae from bronchoscope washing machines. / Nomura, Kazuyo; Ogawa, Midori; Miyamoto, Hiroshi; Muratani, Tetsuro; Taniguchi, Hatsumi.

In: American Journal of Infection Control, Vol. 32, No. 4, 01.06.2004, p. 185-188.

Research output: Contribution to journalArticle

Nomura, Kazuyo ; Ogawa, Midori ; Miyamoto, Hiroshi ; Muratani, Tetsuro ; Taniguchi, Hatsumi. / Antibiotic susceptibility of glutaraldehyde-tolerant Mycobacterium chelonae from bronchoscope washing machines. In: American Journal of Infection Control. 2004 ; Vol. 32, No. 4. pp. 185-188.
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abstract = "Background Contamination of bronchoalveolar lavage fluid is a major problem in the world. Although 2{\%} glutaraldehyde (GA) is widely used as a disinfectant for bronchoscope cleaning, recently, GA-tolerant mycobacteria have been isolated, which makes this problem more complicated. Methods We studied the susceptibility to GA and antibiotics of mycobacteria isolated from bronchoscope washing machines in our hospital. We also studied the minimum inhibitory concentrations of GA and antibiotics with pump inhibitors. Results Twenty-nine mycobacteria were isolated, of which 26 were Mycobacterium chelonae. Among 18 isolates of M chelonae, excluding 8 isolates in which some results were not reproducible, 50{\%} (9 of 18) were 2{\%} GA-tolerant. One hundred percent (9 of 9) of the GA-tolerant isolates and 11{\%} (1 of 9) of the GA-sensitive isolates were either resistant or intermediately resistant to 2 or 3 classes of antibiotics. Efflux pump inhibitors did not influence the susceptibility to GA and antibiotics. Conclusions It was suggested that there might be an association of GA tolerance with antibiotic resistance in M chelonae. There may a different mechanism(s) other than that involving efflux pumps with regard to GA tolerance and antibiotic resistance in M chelonae. When bronchoscopy-related mycobacterial infections are suspected, physicians and clinical microbiologists should exercise care in handling GA-tolerant mycobacteria, which may be resistant to multiple antibiotics.",
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