Antibody titers to HTLV-I-p40tax protein and gag-env hybrid protein in HTLV-I-associated myelopathy/tropical spastic paraparesis: Correlation with increased HTLV-I proviral DNA load

Jun ichi Kira, Minoru Nakamura, Takashi Sawada, Yoshio Koyanagi, Nobuhira Ohori, Yasuto Itoyama, Naoki Yamamoto, Yoshiyuki Sakaki, Ikuo Goto

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Abstract

We studied the relationship between antibody titers to recombinant HTLV-I p40tax protein and gag-env hybrid protein in serum (by an enzyme-linked immunosorbent assay) and HTLV-I proviral DNA load in peripheral blood mononuclear cells (by a quantitative polymerase chain reaction method) in 18 patients with HTLV-I-associated myelopathy (HAM)/tropical spastic paraparesis (TSP), 17 HTLV-I carriers without HAM/TSP and 16 HTLV-I uninfected controls. The IgG and IgA antibody titers to either of the proteins correlated significantly with the HTLV-I pX (coding p40tax protein) and pol DNA amounts in HTLV-I infected subjects. HAM/TSP patients had significantly higher titers of IgG and IgA antibodies to the HTLV-I proteins than did the HTLV-I carriers without HAM/TSP. While the IgM antibodies to the HTLV-I proteins were found in only 6% of HTLV-I carriers without HAM/TSP, they were found in 40% of HAM/TSP patients, especially those having both a high HTLV-I proviral DNA load and high titers of the IgG and IgA antibodies. HAM/TSP patients with the IgM antibodies had a tendency to deteriorate more frequently on the Kurtzke's disability status scale and magnetic resonance imaging of the brain (leukoencephalopathy) than did those without in the two-year follow-up. Thus, the presence of IgM antibody and high titers of IgG and IgA antibodies to the HTLV-I proteins, together with the increased HTLV-I proviral DNA load, appears to distinguish HAM/TSP patients from HTLV-I carriers without HAM/TSP.

Original languageEnglish
Pages (from-to)98-104
Number of pages7
JournalJournal of the Neurological Sciences
Volume107
Issue number1
DOIs
Publication statusPublished - Jan 1992

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Tropical Spastic Paraparesis
env Gene Products
gag Gene Products
Human T-lymphotropic virus 1
Antibodies
DNA
HTLV-I Antibodies
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Proteins
pol Gene Products
Leukoencephalopathies
Blood Cells

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Antibody titers to HTLV-I-p40tax protein and gag-env hybrid protein in HTLV-I-associated myelopathy/tropical spastic paraparesis : Correlation with increased HTLV-I proviral DNA load. / Kira, Jun ichi; Nakamura, Minoru; Sawada, Takashi; Koyanagi, Yoshio; Ohori, Nobuhira; Itoyama, Yasuto; Yamamoto, Naoki; Sakaki, Yoshiyuki; Goto, Ikuo.

In: Journal of the Neurological Sciences, Vol. 107, No. 1, 01.1992, p. 98-104.

Research output: Contribution to journalArticle

Kira, Jun ichi ; Nakamura, Minoru ; Sawada, Takashi ; Koyanagi, Yoshio ; Ohori, Nobuhira ; Itoyama, Yasuto ; Yamamoto, Naoki ; Sakaki, Yoshiyuki ; Goto, Ikuo. / Antibody titers to HTLV-I-p40tax protein and gag-env hybrid protein in HTLV-I-associated myelopathy/tropical spastic paraparesis : Correlation with increased HTLV-I proviral DNA load. In: Journal of the Neurological Sciences. 1992 ; Vol. 107, No. 1. pp. 98-104.
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abstract = "We studied the relationship between antibody titers to recombinant HTLV-I p40tax protein and gag-env hybrid protein in serum (by an enzyme-linked immunosorbent assay) and HTLV-I proviral DNA load in peripheral blood mononuclear cells (by a quantitative polymerase chain reaction method) in 18 patients with HTLV-I-associated myelopathy (HAM)/tropical spastic paraparesis (TSP), 17 HTLV-I carriers without HAM/TSP and 16 HTLV-I uninfected controls. The IgG and IgA antibody titers to either of the proteins correlated significantly with the HTLV-I pX (coding p40tax protein) and pol DNA amounts in HTLV-I infected subjects. HAM/TSP patients had significantly higher titers of IgG and IgA antibodies to the HTLV-I proteins than did the HTLV-I carriers without HAM/TSP. While the IgM antibodies to the HTLV-I proteins were found in only 6{\%} of HTLV-I carriers without HAM/TSP, they were found in 40{\%} of HAM/TSP patients, especially those having both a high HTLV-I proviral DNA load and high titers of the IgG and IgA antibodies. HAM/TSP patients with the IgM antibodies had a tendency to deteriorate more frequently on the Kurtzke's disability status scale and magnetic resonance imaging of the brain (leukoencephalopathy) than did those without in the two-year follow-up. Thus, the presence of IgM antibody and high titers of IgG and IgA antibodies to the HTLV-I proteins, together with the increased HTLV-I proviral DNA load, appears to distinguish HAM/TSP patients from HTLV-I carriers without HAM/TSP.",
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AU - Sawada, Takashi

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AU - Ohori, Nobuhira

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AU - Yamamoto, Naoki

AU - Sakaki, Yoshiyuki

AU - Goto, Ikuo

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AB - We studied the relationship between antibody titers to recombinant HTLV-I p40tax protein and gag-env hybrid protein in serum (by an enzyme-linked immunosorbent assay) and HTLV-I proviral DNA load in peripheral blood mononuclear cells (by a quantitative polymerase chain reaction method) in 18 patients with HTLV-I-associated myelopathy (HAM)/tropical spastic paraparesis (TSP), 17 HTLV-I carriers without HAM/TSP and 16 HTLV-I uninfected controls. The IgG and IgA antibody titers to either of the proteins correlated significantly with the HTLV-I pX (coding p40tax protein) and pol DNA amounts in HTLV-I infected subjects. HAM/TSP patients had significantly higher titers of IgG and IgA antibodies to the HTLV-I proteins than did the HTLV-I carriers without HAM/TSP. While the IgM antibodies to the HTLV-I proteins were found in only 6% of HTLV-I carriers without HAM/TSP, they were found in 40% of HAM/TSP patients, especially those having both a high HTLV-I proviral DNA load and high titers of the IgG and IgA antibodies. HAM/TSP patients with the IgM antibodies had a tendency to deteriorate more frequently on the Kurtzke's disability status scale and magnetic resonance imaging of the brain (leukoencephalopathy) than did those without in the two-year follow-up. Thus, the presence of IgM antibody and high titers of IgG and IgA antibodies to the HTLV-I proteins, together with the increased HTLV-I proviral DNA load, appears to distinguish HAM/TSP patients from HTLV-I carriers without HAM/TSP.

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