TY - JOUR
T1 - Antidermatophyte and antimelanogenesis compound from Eleutherine americana grown in Indonesia
AU - Kusuma, Irawan Wijaya
AU - Arung, Enos Tangke
AU - Rosamah, Enih
AU - Purwatiningsih, Sri
AU - Kuspradini, Harlinda
AU - Syafrizal,
AU - Astuti, Juli
AU - Kim, Yong Ung
AU - Shimizu, Kuniyoshi
N1 - Funding Information:
Acknowledgments This study was supported by a Competitive Research Grant (PHB 2009) program from the Directorate General of Higher Education, Indonesian Ministry of National Education. PT. Kaltim Methanol Industri, Bontang, East Kalimantan, Indonesia, is gratefully acknowledged for providing some chemicals.
PY - 2010/4
Y1 - 2010/4
N2 - An active compound from the bulb of Eleutherine americana L. Merr. (Iridaceae) collected from East Kalimantan, Indonesia, was tested for its antidermatophyte and antimelanogenesis activity. Antifungal assay-directed fractionation of the n-hexane-soluble fraction of the methanolic extract of the bulb of E. americana led to the isolation of 1 as an active compound. The compound was identified as the naphthoquinone eleutherin by EI-MS and 1H-, 13C-, and two-dimensional NMR analyses. Antidermatophyte assay of 1 at concentrations of 10, 20, 40, 60, and 80 μg/disk and myconazole, a commercial antidermatophyte, at 10 μg/disk displayed 7, 8, 13, 16, 17, and 14 mm of inhibition zone against Trichophyton mentagrophytes, respectively. In a melanin formation inhibition assay, compound 1 displayed potent antimelanogenesis activity at 5 ppm with low toxicity compared with arbutin, a commercial skin-whitening agent. The results showed the high potential of 1, an active compound from E. americana, to be applied as an antidermatophyte and antimelanogenesis agent.
AB - An active compound from the bulb of Eleutherine americana L. Merr. (Iridaceae) collected from East Kalimantan, Indonesia, was tested for its antidermatophyte and antimelanogenesis activity. Antifungal assay-directed fractionation of the n-hexane-soluble fraction of the methanolic extract of the bulb of E. americana led to the isolation of 1 as an active compound. The compound was identified as the naphthoquinone eleutherin by EI-MS and 1H-, 13C-, and two-dimensional NMR analyses. Antidermatophyte assay of 1 at concentrations of 10, 20, 40, 60, and 80 μg/disk and myconazole, a commercial antidermatophyte, at 10 μg/disk displayed 7, 8, 13, 16, 17, and 14 mm of inhibition zone against Trichophyton mentagrophytes, respectively. In a melanin formation inhibition assay, compound 1 displayed potent antimelanogenesis activity at 5 ppm with low toxicity compared with arbutin, a commercial skin-whitening agent. The results showed the high potential of 1, an active compound from E. americana, to be applied as an antidermatophyte and antimelanogenesis agent.
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U2 - 10.1007/s11418-010-0396-7
DO - 10.1007/s11418-010-0396-7
M3 - Article
C2 - 20155402
AN - SCOPUS:77953564693
SN - 1340-3443
VL - 64
SP - 223
EP - 226
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 2
ER -