Antifungal activity of rye (secale cereale) seed chitinases

The different binding manner of class i and class ii chitinases to the fungal cell walls

Toki Taira, Takayuki Ohnuma, Takeshi Yamagami, Yoichi Aso, Masatsune Ishiguro, Masanobu Ishihara

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The antifungal activities of rye seed chitinase-a (RSC-a, class I) and -c (RSC-c, class II) were studied in detail using two different bioassays with Trichoderma sp. as well as binding and degradation experiments with the cell walls prepared from its mycelia. RSC-a inhibited more strongly the re-extension of the hyphae, containing mainly mature cells, than RSC-c did. Upon incubation of the fungus with fluorescent chitinases, FITC- labeled RSC-a was found to be located in the hyphal tips, lateral walls, and septa, while FITC-labeled RSC-c was only in the hyphal tip. RSC-a had a greater affinity for the cell walls than RSC-c. RSC-a liberated a larger amount of reducing sugar from the cell walls than RSC-c did. These results inferred that RSC-a first binds to the lateral walls and septa, consisting of the mature cell walls, and degrades mature chitin fiber, while RSC-c binds only to the hyphal tip followed by degradation of only nascent chitin. As a result, RSC-a inhibited fungal growth more effectively than RSC-c. Furthermore, it was suggested that the chitin-binding domain in RSC-a assists the antifungal action of RSC-a by binding to the fungal hypha.

Original languageEnglish
Pages (from-to)970-977
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number5
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Chitinases
Cell Wall
Chitin
Seed
Seeds
Cells
Hyphae
Fluorescein-5-isothiocyanate
Degradation
Trichoderma
Bioassay
Mycelium
Fungi
Sugars
Biological Assay
Secale
Fibers
Growth
Experiments

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Antifungal activity of rye (secale cereale) seed chitinases : The different binding manner of class i and class ii chitinases to the fungal cell walls. / Taira, Toki; Ohnuma, Takayuki; Yamagami, Takeshi; Aso, Yoichi; Ishiguro, Masatsune; Ishihara, Masanobu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 5, 01.01.2002, p. 970-977.

Research output: Contribution to journalArticle

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