Antigen delivery targeted to tumor-associated macrophages overcomes tumor immune resistance

Daisuke Muraoka, Naohiro Seo, Tae Hayashi, Yoshiro Tahara, Keisuke Fujii, Isao Tawara, Yoshihiro Miyahara, Kana Okamori, Hideo Yagita, Seiya Imoto, Rui Yamaguchi, Mitsuhiro Komura, Satoru Miyano, Masahiro Goto, Shin Ichi Sawada, Akira Asai, Hiroaki Ikeda, Kazunari Akiyoshi, Naozumi Harada, Hiroshi Shiku

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Immune checkpoint inhibitors and adoptive transfer of gene-engineered T cells have emerged as novel therapeutic modalities for hard-to-treat solid tumors; however, many patients are refractory to these immunotherapies, and the mechanisms underlying tumor immune resistance have not been fully elucidated. By comparing the tumor microenvironment of checkpoint inhibition–sensitive and –resistant murine solid tumors, we observed that the resistant tumors had low immunogenicity. We identified antigen presentation by CD11b + F4/80 + tumor–associated macrophages (TAMs) as a key factor correlated with immune resistance. In the resistant tumors, TAMs remained inactive and did not exert antigen-presenting activity. Targeted delivery of a long peptide antigen to TAMs by using a nano-sized hydrogel (nanogel) in the presence of a TLR agonist activated TAMs, induced their antigen-presenting activity, and thereby transformed the resistant tumors into tumors sensitive to adaptive immune responses such as adoptive transfer of tumor-specific T cell receptor–engineered T cells. These results indicate that the status and function of TAMs have a significant impact on tumor immune sensitivity and that manipulation of TAM functions would be an effective approach for improving the efficacy of immunotherapies.

Original languageEnglish
Pages (from-to)1278-1294
Number of pages17
JournalJournal of Clinical Investigation
Volume129
Issue number3
DOIs
Publication statusPublished - Mar 2019

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Muraoka, D., Seo, N., Hayashi, T., Tahara, Y., Fujii, K., Tawara, I., Miyahara, Y., Okamori, K., Yagita, H., Imoto, S., Yamaguchi, R., Komura, M., Miyano, S., Goto, M., Sawada, S. I., Asai, A., Ikeda, H., Akiyoshi, K., Harada, N., & Shiku, H. (2019). Antigen delivery targeted to tumor-associated macrophages overcomes tumor immune resistance. Journal of Clinical Investigation, 129(3), 1278-1294. https://doi.org/10.1172/JCI97642