Antigen-specific in vitro immunization: A source for human monoclonal antibodies

Kosuke Tomimatsu, Sanetaka Shirahata

    Research output: Chapter in Book/Report/Conference proceedingChapter

    2 Citations (Scopus)


    Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.

    Original languageEnglish
    Title of host publicationHuman Monoclonal Antibodies
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press Inc.
    Number of pages11
    ISBN (Print)9781627035859
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745

    All Science Journal Classification (ASJC) codes

    • Molecular Biology
    • Genetics


    Dive into the research topics of 'Antigen-specific in vitro immunization: A source for human monoclonal antibodies'. Together they form a unique fingerprint.

    Cite this