Antigen-specific in vitro immunization: A source for human monoclonal antibodies

Kosuke Tomimatsu; Sanetaka Shirahata

doi:10.1007/978-1-62703-586-6_15

Antigen-specific in vitro immunization: A source for human monoclonal antibodies

Kosuke Tomimatsu, Sanetaka Shirahata

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.

Original language	English
Title of host publication	Human Monoclonal Antibodies
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	297-307
Number of pages	11
ISBN (Print)	9781627035859
DOIs	https://doi.org/10.1007/978-1-62703-586-6_15
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1060
ISSN (Print)	1064-3745

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-62703-586-6_15

Cite this

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abstract = "Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.",

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AU - Shirahata, Sanetaka

PY - 2014

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AB - Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.

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BT - Human Monoclonal Antibodies

PB - Humana Press Inc.

ER -

Antigen-specific in vitro immunization: A source for human monoclonal antibodies

Abstract

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All Science Journal Classification (ASJC) codes

UN SDGs

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