Antigen-specific suppressor T lymphocytes (Leu-2a+3a-) in human Schistosomiasis japonica

N. Ohta, M. Minai, Takehiko Sasazuki

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Abstract

Immunoregulatory mechanisms in human schistosomiasis japonica were investigated through in vitro T lymphocyte-macrophage co-culture experiments. High responder T lymphocytes responded to the schistosomal adult worm antigen in the presence of HLA-DR-compatible low responder macrophages, whereas low responder T lymphocytes failed to respond to this antigen even in the presence of HLA-DR-compatible high responder macrophages. This observation strongly suggested that T lymphocytes, but not macrophages, controlled the low responsiveness to the schistosomal adult worm antigen. When high responder lymphocytes were challenged with the schistosomal antigen in the presence of low responder T lymphocytes, low responder T lymphocytes caused an antigen-specific suppression, in a dose-dependent manner, of the responsiveness of high responder lymphocytes. The suppressor T lymphocytes were mitomycin C resistant and were positive for Leu-2a, but were negative for Leu-3a. When Leu-2a+ T lymphocytes were depleted from low responder T lymphocytes, a vigorous response by the Leu-2a-depleted T lymphocytes (Leu-2a-3a+) to the schistosomal adult worm antigen was observed. By additing back the Leu-2a+ T lymphocytes into autologous T lymphocyte subpopulations, the specific response to the adult worm antigen was completely suppressed. These findings clearly demonstrate the existence of Leu-2a+3a- suppressor T lymphocytes that can control low responsiveness to the schistosomal adult worm antigen in man.

Original languageEnglish
Pages (from-to)2524-2528
Number of pages5
JournalJournal of Immunology
Volume131
Issue number5
Publication statusPublished - 1983
Externally publishedYes

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Schistosomiasis japonica
T-Lymphocytes
Antigens
Macrophages
HLA-DR Antigens
Lymphocytes
Lymphocyte Subsets
Mitomycin
Coculture Techniques

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Antigen-specific suppressor T lymphocytes (Leu-2a+3a-) in human Schistosomiasis japonica. / Ohta, N.; Minai, M.; Sasazuki, Takehiko.

In: Journal of Immunology, Vol. 131, No. 5, 1983, p. 2524-2528.

Research output: Contribution to journalArticle

Ohta, N, Minai, M & Sasazuki, T 1983, 'Antigen-specific suppressor T lymphocytes (Leu-2a+3a-) in human Schistosomiasis japonica', Journal of Immunology, vol. 131, no. 5, pp. 2524-2528.
Ohta, N. ; Minai, M. ; Sasazuki, Takehiko. / Antigen-specific suppressor T lymphocytes (Leu-2a+3a-) in human Schistosomiasis japonica. In: Journal of Immunology. 1983 ; Vol. 131, No. 5. pp. 2524-2528.
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