Antigenic Determinants on Fimbriae of Serratia marcescens US5 Analyzed Using Monoclonal Antibodies

Seiya Jingushi, Masao Mitsuyama, Tetsuhiro Moriya, Kazunobu Amako, Shun-Ichiro Kawabata, Sadaaki Iwanaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The antigenic sites on small thin fimbriae of Serratia marcescens strain US5 were investigated using immunoelectron microscopy and monoclonal antibodies (MAbs). Negative staining of the fimbriae after treatment with MAbs showed a regularly spaced arrangement of the antibody molecules. When the subunit peptide was subjected to immunoblotting using the MAbs, a single band with a molecular weight of approximately 19kD was evident. This binding of the MAbs to the sub-unit peptide was completely abrogated after treatment with 2-mercaptoethanol, thereby suggesting the important role of disulfide linkage in the maintenance of the conformation of the antigenic site reacted with MAbs. Amino acid analysis of the subunit peptide revealed two cysteine residues, and cysteine residues were absent in the N-terminal portion.

Original languageEnglish
Pages (from-to)879-889
Number of pages11
JournalMICROBIOLOGY and IMMUNOLOGY
Volume31
Issue number9
DOIs
Publication statusPublished - Jan 1 1987

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Serratia marcescens
Epitopes
Monoclonal Antibodies
Peptides
Cysteine
Negative Staining
Immunoelectron Microscopy
Mercaptoethanol
Immunoblotting
Disulfides
Molecular Weight
Maintenance
Amino Acids
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Antigenic Determinants on Fimbriae of Serratia marcescens US5 Analyzed Using Monoclonal Antibodies. / Jingushi, Seiya; Mitsuyama, Masao; Moriya, Tetsuhiro; Amako, Kazunobu; Kawabata, Shun-Ichiro; Iwanaga, Sadaaki.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 31, No. 9, 01.01.1987, p. 879-889.

Research output: Contribution to journalArticle

Jingushi, Seiya ; Mitsuyama, Masao ; Moriya, Tetsuhiro ; Amako, Kazunobu ; Kawabata, Shun-Ichiro ; Iwanaga, Sadaaki. / Antigenic Determinants on Fimbriae of Serratia marcescens US5 Analyzed Using Monoclonal Antibodies. In: MICROBIOLOGY and IMMUNOLOGY. 1987 ; Vol. 31, No. 9. pp. 879-889.
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