Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract

Enos Tangke Arung, Irawan Wijaya Kusuma, Sri Purwatiningsih, Seong Soo Roh, Chae Ha Yang, Soohyeon Jeon, Yong Ung Kim, Edi Sukaton, Joko Susilo, Yuli Astuti, Britanto Dani Wicaksono, Ferry Sandra, Kuniyoshi Shimizu, Ryuichiro Kondo

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Abstract

We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50μg/mL, 87.5μg/mL, and 125μg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.

Original languageEnglish
Pages (from-to)306-308
Number of pages3
JournalJAMS Journal of Acupuncture and Meridian Studies
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 1 2009

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Traditional Medicine
Methanol
Antioxidants
Liver Neoplasms
Ether
Phytotherapy
Indonesia
Hep G2 Cells
Medicinal Plants
Ascorbic Acid
Liver Diseases
ethyl acetate
2,2-diphenyl-1-picrylhydrazyl
n-hexane

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine
  • Anesthesiology and Pain Medicine

Cite this

Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract. / Arung, Enos Tangke; Kusuma, Irawan Wijaya; Purwatiningsih, Sri; Roh, Seong Soo; Yang, Chae Ha; Jeon, Soohyeon; Kim, Yong Ung; Sukaton, Edi; Susilo, Joko; Astuti, Yuli; Wicaksono, Britanto Dani; Sandra, Ferry; Shimizu, Kuniyoshi; Kondo, Ryuichiro.

In: JAMS Journal of Acupuncture and Meridian Studies, Vol. 2, No. 4, 01.12.2009, p. 306-308.

Research output: Contribution to journalArticle

Arung, ET, Kusuma, IW, Purwatiningsih, S, Roh, SS, Yang, CH, Jeon, S, Kim, YU, Sukaton, E, Susilo, J, Astuti, Y, Wicaksono, BD, Sandra, F, Shimizu, K & Kondo, R 2009, 'Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract', JAMS Journal of Acupuncture and Meridian Studies, vol. 2, no. 4, pp. 306-308. https://doi.org/10.1016/S2005-2901(09)60073-X
Arung, Enos Tangke ; Kusuma, Irawan Wijaya ; Purwatiningsih, Sri ; Roh, Seong Soo ; Yang, Chae Ha ; Jeon, Soohyeon ; Kim, Yong Ung ; Sukaton, Edi ; Susilo, Joko ; Astuti, Yuli ; Wicaksono, Britanto Dani ; Sandra, Ferry ; Shimizu, Kuniyoshi ; Kondo, Ryuichiro. / Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract. In: JAMS Journal of Acupuncture and Meridian Studies. 2009 ; Vol. 2, No. 4. pp. 306-308.
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