Abstract
Oxidative stress causes cell death and induces many kinds of disease, including liver disease. Nitroxides are known to react catalytically with free radicals. In this study, the cell protective activities of nitroxides were compared with those of other antioxidants. Nitroxides showed much greater inhibition of hydrogen peroxide-induced cell death than other antioxidants in a hepatic cell line and in primary hepatocytes. The intracellular oxidative stress level at 24 h after hydrogen peroxide stimulation was significantly decreased by nitroxides, but not by other antioxidants. To clarify the mechanism of cell protection by nitroxides, we investigated whether nitroxides inhibited DNA damage and mitogen-activated protein kinase pathway activation. We found that nitroxides reduced caspase3 activation and may have ultimately inhibited cell death. In conclusion, nitroxides are very useful for attenuating cell damage due to oxidative stress. Nitroxides are thus a potential therapeutic agent for oxidative stress-related diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 132-138 |
| Number of pages | 7 |
| Journal | Journal of Clinical Biochemistry and Nutrition |
| Volume | 62 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 2018 |
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All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Nutrition and Dietetics
- Clinical Biochemistry
Cite this
Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death. / Shinto, Saki; Matsuoka, Yuta; Yamato, Mayumi; Yamada, Ken Ichi.
In: Journal of Clinical Biochemistry and Nutrition, Vol. 62, No. 2, 03.2018, p. 132-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death
AU - Shinto, Saki
AU - Matsuoka, Yuta
AU - Yamato, Mayumi
AU - Yamada, Ken Ichi
PY - 2018/3
Y1 - 2018/3
N2 - Oxidative stress causes cell death and induces many kinds of disease, including liver disease. Nitroxides are known to react catalytically with free radicals. In this study, the cell protective activities of nitroxides were compared with those of other antioxidants. Nitroxides showed much greater inhibition of hydrogen peroxide-induced cell death than other antioxidants in a hepatic cell line and in primary hepatocytes. The intracellular oxidative stress level at 24 h after hydrogen peroxide stimulation was significantly decreased by nitroxides, but not by other antioxidants. To clarify the mechanism of cell protection by nitroxides, we investigated whether nitroxides inhibited DNA damage and mitogen-activated protein kinase pathway activation. We found that nitroxides reduced caspase3 activation and may have ultimately inhibited cell death. In conclusion, nitroxides are very useful for attenuating cell damage due to oxidative stress. Nitroxides are thus a potential therapeutic agent for oxidative stress-related diseases.
AB - Oxidative stress causes cell death and induces many kinds of disease, including liver disease. Nitroxides are known to react catalytically with free radicals. In this study, the cell protective activities of nitroxides were compared with those of other antioxidants. Nitroxides showed much greater inhibition of hydrogen peroxide-induced cell death than other antioxidants in a hepatic cell line and in primary hepatocytes. The intracellular oxidative stress level at 24 h after hydrogen peroxide stimulation was significantly decreased by nitroxides, but not by other antioxidants. To clarify the mechanism of cell protection by nitroxides, we investigated whether nitroxides inhibited DNA damage and mitogen-activated protein kinase pathway activation. We found that nitroxides reduced caspase3 activation and may have ultimately inhibited cell death. In conclusion, nitroxides are very useful for attenuating cell damage due to oxidative stress. Nitroxides are thus a potential therapeutic agent for oxidative stress-related diseases.
UR - http://www.scopus.com/inward/record.url?scp=85043532874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85043532874&partnerID=8YFLogxK
U2 - 10.3164/jcbn.17-60
DO - 10.3164/jcbn.17-60
M3 - Article
AN - SCOPUS:85043532874
VL - 62
SP - 132
EP - 138
JO - Journal of Clinical Biochemistry and Nutrition
JF - Journal of Clinical Biochemistry and Nutrition
SN - 0912-0009
IS - 2
ER -