Antisense inhibition of vascular endothelial growth factor in human head and neck squamous cell carcinoma

Torahiko Nakashima, J. Michael Hudson, Gary L. Clayman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background. Vascular endothelial growth factor (VEGF) is a potent paracrine angiogenic factor involved in angiogenesis. We determined whether antisense VEGF transfection can suppress angiogenic activity of a human squamous cell carcinoma of the head and neck (SCCHN) cell line. Methods. Human SCCHN cell lines were screened for VEGF secretion by ELISA. The highest VEGF secreting cell line was transfected with an antisense VEGF vector. Endothelial cell migration assays were performed using the conditioned medium from the transfected clones. Tumorigenicity assays of the transfectants in nude mice were also performed. Results. Antisense VEGF expression exhibited a 20-fold inhibition of VEGF secretion. The addition of conditioned medium from the antisense clones resulted in 50% reduction of endothelial migration. There was no effect on in vivo tumorigenicity. Conclusions. Antisense VEGF transfection effectively downregulated VEGF secretion from SCCHN cells that had high VEGF secretion. Targeting VEGF expression may be useful for suppressing angiogenesis in head and neck cancer. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish
Pages (from-to)483-488
Number of pages6
JournalHead and Neck
Volume22
Issue number5
DOIs
Publication statusPublished - Aug 2000

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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