TY - JOUR
T1 - Apoptosis and p53 expression in adenocarcinoma and squamous cell carcinoma of the lung
AU - Kunitake, Ritsuko
AU - Kuwano, Kazuyoshi
AU - Kawasaki, Masayuki
AU - Hagimoto, Naoki
AU - Nomoto, Yoshitsugu
AU - Nakanishi, Yoichi
AU - Mitsutomi, Tetsuya
AU - Hara, Nobuyuki
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/6
Y1 - 1996/6
N2 - We investigated the association between DNA damage or apoptosis and the expression of p53 protein in untreated adenocarcinoma and squamous cell carcinoma of the lung, using the method of terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling (TUNEL) and immunohistochemistry (IHC) for p53 protein, respectively. We performed IHC and TUNEL on formalin-fixed, paraffin-embedded tissues from 23 cases of lung cancer (11 of adenocarcinoma and 12 of squamous cell carcinoma) obtained by lobectomy. The results showed that TUNEL-positive cells were detected in 6 of 23 cases (26%), and p53 proteins were detected in 12 of 23 cases (52%). There was no significant difference in the number of p53 positive cases in TUNEL- positive cases (4/6 cases) or in TUNEL-negative cases (8/17 cases). There was no direct association between apoptosis and the expression of p53 in this study. We speculate it may be due to the presence of other factors and pathways in the process of apoptosis.
AB - We investigated the association between DNA damage or apoptosis and the expression of p53 protein in untreated adenocarcinoma and squamous cell carcinoma of the lung, using the method of terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling (TUNEL) and immunohistochemistry (IHC) for p53 protein, respectively. We performed IHC and TUNEL on formalin-fixed, paraffin-embedded tissues from 23 cases of lung cancer (11 of adenocarcinoma and 12 of squamous cell carcinoma) obtained by lobectomy. The results showed that TUNEL-positive cells were detected in 6 of 23 cases (26%), and p53 proteins were detected in 12 of 23 cases (52%). There was no significant difference in the number of p53 positive cases in TUNEL- positive cases (4/6 cases) or in TUNEL-negative cases (8/17 cases). There was no direct association between apoptosis and the expression of p53 in this study. We speculate it may be due to the presence of other factors and pathways in the process of apoptosis.
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U2 - 10.2482/haigan.36.271
DO - 10.2482/haigan.36.271
M3 - Article
AN - SCOPUS:0030016965
SN - 0386-9628
VL - 36
SP - 271
EP - 276
JO - Japanese Journal of Lung Cancer
JF - Japanese Journal of Lung Cancer
IS - 3
ER -
