Apoptosis and p53 expression in adenocarcinoma and squamous cell carcinoma of the lung

Ritsuko Kunitake, Kazuyoshi Kuwano, Masayuki Kawasaki, Naoki Hagimoto, Yoshitsugu Nomoto, Yoichi Nakanishi, Tetsuya Mitsutomi, Nobuyuki Hara

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the association between DNA damage or apoptosis and the expression of p53 protein in untreated adenocarcinoma and squamous cell carcinoma of the lung, using the method of terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling (TUNEL) and immunohistochemistry (IHC) for p53 protein, respectively. We performed IHC and TUNEL on formalin-fixed, paraffin-embedded tissues from 23 cases of lung cancer (11 of adenocarcinoma and 12 of squamous cell carcinoma) obtained by lobectomy. The results showed that TUNEL-positive cells were detected in 6 of 23 cases (26%), and p53 proteins were detected in 12 of 23 cases (52%). There was no significant difference in the number of p53 positive cases in TUNEL- positive cases (4/6 cases) or in TUNEL-negative cases (8/17 cases). There was no direct association between apoptosis and the expression of p53 in this study. We speculate it may be due to the presence of other factors and pathways in the process of apoptosis.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalJapanese Journal of Lung Cancer
Volume36
Issue number3
DOIs
Publication statusPublished - Jun 1996

All Science Journal Classification (ASJC) codes

  • Oncology
  • Pulmonary and Respiratory Medicine

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