TY - JOUR
T1 - Apoptosis in perforated cornea of a patient with graft-versus-host disease
AU - Yoshida, Ayako
AU - Kawano, Yoh Ichi
AU - Kato, Kouji
AU - Yoshida, Shigeo
AU - Yoshikawa, Hiroshi
AU - Muta, Tsuyoshi
AU - Eto, Tetsuya
AU - Gondo, Hisashi
AU - Shibuya, Tsunefumi
AU - Ishibashi, Tatsuro
AU - Yamana, Toshiko
PY - 2006/8
Y1 - 2006/8
N2 - Case report: Although ocular complications associated with graft-versus-host disease (GVHD) can include corneal dysfunction, corneal perforation is not common. We report the presence of apoptotic cells in a perforated cornea of a patient with GVHD. A 72-year-old man with the angioimmunoblastic type of malignant lymphoma developed chronic GVHD after allogeneic peripheral blood stem cell transplantation. Despite systemic and topical treatment, both corneas perforated, and penetrating keratoplasty with cataract extraction and intraocular lens implantation was performed on both eyes. Comments: The corneal button excised from the right eye was examined histologically and stained for apoptotic cells by TdT-mediated dUTP nick end labeling (TUNEL). This revealed thinning of the epithelial cell layer and stroma, with cells, including lymphocytes, infiltrating to the site of the perforation. Some of the epithelial cells and keratocytes were TUNEL positive. The presence of apoptotic cells in our case suggests that apoptosis may be involved in the perforation of the cornea in patients with GVHD.
AB - Case report: Although ocular complications associated with graft-versus-host disease (GVHD) can include corneal dysfunction, corneal perforation is not common. We report the presence of apoptotic cells in a perforated cornea of a patient with GVHD. A 72-year-old man with the angioimmunoblastic type of malignant lymphoma developed chronic GVHD after allogeneic peripheral blood stem cell transplantation. Despite systemic and topical treatment, both corneas perforated, and penetrating keratoplasty with cataract extraction and intraocular lens implantation was performed on both eyes. Comments: The corneal button excised from the right eye was examined histologically and stained for apoptotic cells by TdT-mediated dUTP nick end labeling (TUNEL). This revealed thinning of the epithelial cell layer and stroma, with cells, including lymphocytes, infiltrating to the site of the perforation. Some of the epithelial cells and keratocytes were TUNEL positive. The presence of apoptotic cells in our case suggests that apoptosis may be involved in the perforation of the cornea in patients with GVHD.
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U2 - 10.1016/S0008-4182(06)80010-4
DO - 10.1016/S0008-4182(06)80010-4
M3 - Article
C2 - 16883364
AN - SCOPUS:33748059544
SN - 0008-4182
VL - 41
SP - 472
EP - 475
JO - Transactions of the Canadian Ophthalmological Society
JF - Transactions of the Canadian Ophthalmological Society
IS - 4
ER -