Apoptosis-inducing factor, mitochondrion-associated 2, regulates Klf1 in a mouse erythroleukemia cell line

Naoko Kojima, Yuka Tanaka, Kasem Kulkeaw, Yoichi Nakanishi, Senji Shirasawa, Daisuke Sugiyama

Research output: Contribution to journalArticle

Abstract

Background/Aim: Apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), is a DNA-binding oxoreductase protein that promotes apoptosis. To assess its potential role in erythropoiesis we analyzed the effects of Aifm2 loss-of-function in the murine erythroleukemia line (MEL). Materials and Methods: MEL cells were transfected with siRNA targeting Aifm2 for 24 h and evaluated by cell counting, flow cytometry with annexin V and PI staining and gene expression analysis. Results: Aifm2 knockdown did not affect the apoptotic status of MEL cells. However, Aifm2 knockdown significantly increased expression of the erythropoietic transcription factor Klf1 (2.9?}0.2-fold, p<0.05) and decreased α- And β-globin expression (0.6?}0.2-fold, p<0.05 and 0.5?}0.2-fold, p<0.01). Conclusion: Aifm2 may function in differentiation of erythroid MEL cells in vitro.

Original languageEnglish
Pages (from-to)4493-4500
Number of pages8
JournalAnticancer research
Volume35
Issue number8
Publication statusPublished - Aug 1 2015

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Apoptosis Inducing Factor
Leukemia, Erythroblastic, Acute
Mitochondria
Cell Line
Globins
Erythropoiesis
Annexin A5
DNA-Binding Proteins
Small Interfering RNA
Flow Cytometry
Transcription Factors
Apoptosis
Staining and Labeling
Gene Expression

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kojima, N., Tanaka, Y., Kulkeaw, K., Nakanishi, Y., Shirasawa, S., & Sugiyama, D. (2015). Apoptosis-inducing factor, mitochondrion-associated 2, regulates Klf1 in a mouse erythroleukemia cell line. Anticancer research, 35(8), 4493-4500.

Apoptosis-inducing factor, mitochondrion-associated 2, regulates Klf1 in a mouse erythroleukemia cell line. / Kojima, Naoko; Tanaka, Yuka; Kulkeaw, Kasem; Nakanishi, Yoichi; Shirasawa, Senji; Sugiyama, Daisuke.

In: Anticancer research, Vol. 35, No. 8, 01.08.2015, p. 4493-4500.

Research output: Contribution to journalArticle

Kojima, N, Tanaka, Y, Kulkeaw, K, Nakanishi, Y, Shirasawa, S & Sugiyama, D 2015, 'Apoptosis-inducing factor, mitochondrion-associated 2, regulates Klf1 in a mouse erythroleukemia cell line', Anticancer research, vol. 35, no. 8, pp. 4493-4500.
Kojima, Naoko ; Tanaka, Yuka ; Kulkeaw, Kasem ; Nakanishi, Yoichi ; Shirasawa, Senji ; Sugiyama, Daisuke. / Apoptosis-inducing factor, mitochondrion-associated 2, regulates Klf1 in a mouse erythroleukemia cell line. In: Anticancer research. 2015 ; Vol. 35, No. 8. pp. 4493-4500.
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