Appearance of extrathymic early differentiated CD4-CD8- T cells with t cell receptor γ/δ or α/β after thymus grafting to nude mice: Influence of thymus on extrathymic t cell differentiation

Hiroyuki Kenai, Yasunobu Yoshikai, Goro Matsuzaki, Akinori Iwasaki, Hiroyuki Yuuki, Takao Nakamura, Kikuo Nomoto

    Research output: Contribution to journalArticle

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    Abstract

    Fetal thymus grafting into athymic nude mice has been used as an experimental model of T cell development. To understand the early events of T cell development, we have examined the sequence of appearance of T cell subsets in lymph nodes (LN) of BALB/c nu/nu mice after grafting with syngeneic fetal thymus. T cells expressing T cell receptor (TCR) α/β or γ/δ increased in LN from 1 week after grafting, although no host-derived CD3+ T cells were detected in the grafted thymus and no donor thymus- derived T cells were detected in the LN. The early appearing T cells of both TCR α/β and TCR γ/δ showed a CD4-CD8- phenotype. V region usage analysis of the early appearing TCR α/β T cells revealed that they contained cells bearing Vβ3 or Vβ11, which are potentially reactive to self-superantigen Mls-2a or Dvb11, respectively, and are deleted in the course of T cell development in the thymus of euthymic BALB/c mice. The early appearing T cells showed neither mixed lymphocyte reaction nor cytotoxic T cell activity against allogeneic cells. In contrast, lymphokine-activated killer cells from early appearing T cells, which contained high percentages of TCR γ/δ T cells, exhibited higher cytotoxic activity against P815 mastocytoma than those from euthymic mice or untreated nude mice. All these results suggest that the early appearing T cells are developed extrathymically. We propose that the thymus may induce extrathymic T cell development without direct cell-to-cell interaction. It seems likely that the extrathymically developed T cells, especially TCR γ/δ T cells, induced by the thymus have some role in the defense mechanism in the absence of conventional thymus-derived T cells.

    Original languageEnglish
    Pages (from-to)79-93
    Number of pages15
    JournalCellular Immunology
    Volume153
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 1994

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    Nude Mice
    Thymus Gland
    Cell Differentiation
    T-Lymphocytes
    T-Cell Antigen Receptor
    Lymph Nodes
    Mastocytoma
    Lymphokine-Activated Killer Cells
    Superantigens
    Mixed Lymphocyte Culture Test
    T-Lymphocyte Subsets
    Cell Communication

    All Science Journal Classification (ASJC) codes

    • Immunology

    Cite this

    Appearance of extrathymic early differentiated CD4-CD8- T cells with t cell receptor γ/δ or α/β after thymus grafting to nude mice : Influence of thymus on extrathymic t cell differentiation. / Kenai, Hiroyuki; Yoshikai, Yasunobu; Matsuzaki, Goro; Iwasaki, Akinori; Yuuki, Hiroyuki; Nakamura, Takao; Nomoto, Kikuo.

    In: Cellular Immunology, Vol. 153, No. 1, 01.01.1994, p. 79-93.

    Research output: Contribution to journalArticle

    Kenai, Hiroyuki ; Yoshikai, Yasunobu ; Matsuzaki, Goro ; Iwasaki, Akinori ; Yuuki, Hiroyuki ; Nakamura, Takao ; Nomoto, Kikuo. / Appearance of extrathymic early differentiated CD4-CD8- T cells with t cell receptor γ/δ or α/β after thymus grafting to nude mice : Influence of thymus on extrathymic t cell differentiation. In: Cellular Immunology. 1994 ; Vol. 153, No. 1. pp. 79-93.
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    abstract = "Fetal thymus grafting into athymic nude mice has been used as an experimental model of T cell development. To understand the early events of T cell development, we have examined the sequence of appearance of T cell subsets in lymph nodes (LN) of BALB/c nu/nu mice after grafting with syngeneic fetal thymus. T cells expressing T cell receptor (TCR) α/β or γ/δ increased in LN from 1 week after grafting, although no host-derived CD3+ T cells were detected in the grafted thymus and no donor thymus- derived T cells were detected in the LN. The early appearing T cells of both TCR α/β and TCR γ/δ showed a CD4-CD8- phenotype. V region usage analysis of the early appearing TCR α/β T cells revealed that they contained cells bearing Vβ3 or Vβ11, which are potentially reactive to self-superantigen Mls-2a or Dvb11, respectively, and are deleted in the course of T cell development in the thymus of euthymic BALB/c mice. The early appearing T cells showed neither mixed lymphocyte reaction nor cytotoxic T cell activity against allogeneic cells. In contrast, lymphokine-activated killer cells from early appearing T cells, which contained high percentages of TCR γ/δ T cells, exhibited higher cytotoxic activity against P815 mastocytoma than those from euthymic mice or untreated nude mice. All these results suggest that the early appearing T cells are developed extrathymically. We propose that the thymus may induce extrathymic T cell development without direct cell-to-cell interaction. It seems likely that the extrathymically developed T cells, especially TCR γ/δ T cells, induced by the thymus have some role in the defense mechanism in the absence of conventional thymus-derived T cells.",
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