Abstract
Thymocytes expressing homogeneous TCR Vγ5/Vδ1 gene without N nucleotide addition (canonical sequence) have been reported to be positively selected in fetal thymus. Because skin intraepithelial lymphocytes (s-IEL) also express canonical sequence, it is proposed that s-IEL are generated by an epidermis- specific homing of these positive selected fetal Vγ5/Vδ1-bearing thymocytes. We have reported that positive selection of Vγ5+ thymocytes is not seen in AKR/J→C3H/He bone marrow chimeras. In this report, we analyzed the phenotype of donor-derived s-IEL from AKR/J→C3H/He bone marrow chimeras that appeared in the absence of positive selection of Vγ5-bearing thymocytes. We found interestingly that almost all donor-derived s-IEL in the bone marrow chimeras expressed TCR-αβ and lack expression of both CD4 and CD8. Furthermore, TCR-αβ+ s-IEL were detected in adult-thymectomized bone marrow chimeras, indicating that the TCR-αβ+ s-IEL are extrathymically derived. All these results suggest that the s-IEL expressing TCR-αβ appear extrathymically in the absence of positive selection of Vγ5/Vδ1-bearing thymocytes. Our results may also explain the contradicting result between human s-IEL, which are TCR-αβ+CD4-CD8- and murine s-IEL, and suggest the importance of extrathymic T cell differentiation pathway of the TCR-αβ lineage in immune surveillance.
| Original language | English |
|---|---|
| Pages (from-to) | 3000-3006 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 151 |
| Issue number | 6 |
| Publication status | Published - 1993 |
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All Science Journal Classification (ASJC) codes
- Immunology
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Appearance of TCR-αβ+CD4-CD8- skin intraepithelial lymphocytes in radiation bone marrow chimeras. / Ogimoto, M.; Matsuzaki, G.; Yoshikai, Y.; Tauchi, Y.; Nomoto, K.
In: Journal of Immunology, Vol. 151, No. 6, 1993, p. 3000-3006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Appearance of TCR-αβ+CD4-CD8- skin intraepithelial lymphocytes in radiation bone marrow chimeras
AU - Ogimoto, M.
AU - Matsuzaki, G.
AU - Yoshikai, Y.
AU - Tauchi, Y.
AU - Nomoto, K.
PY - 1993
Y1 - 1993
N2 - Thymocytes expressing homogeneous TCR Vγ5/Vδ1 gene without N nucleotide addition (canonical sequence) have been reported to be positively selected in fetal thymus. Because skin intraepithelial lymphocytes (s-IEL) also express canonical sequence, it is proposed that s-IEL are generated by an epidermis- specific homing of these positive selected fetal Vγ5/Vδ1-bearing thymocytes. We have reported that positive selection of Vγ5+ thymocytes is not seen in AKR/J→C3H/He bone marrow chimeras. In this report, we analyzed the phenotype of donor-derived s-IEL from AKR/J→C3H/He bone marrow chimeras that appeared in the absence of positive selection of Vγ5-bearing thymocytes. We found interestingly that almost all donor-derived s-IEL in the bone marrow chimeras expressed TCR-αβ and lack expression of both CD4 and CD8. Furthermore, TCR-αβ+ s-IEL were detected in adult-thymectomized bone marrow chimeras, indicating that the TCR-αβ+ s-IEL are extrathymically derived. All these results suggest that the s-IEL expressing TCR-αβ appear extrathymically in the absence of positive selection of Vγ5/Vδ1-bearing thymocytes. Our results may also explain the contradicting result between human s-IEL, which are TCR-αβ+CD4-CD8- and murine s-IEL, and suggest the importance of extrathymic T cell differentiation pathway of the TCR-αβ lineage in immune surveillance.
AB - Thymocytes expressing homogeneous TCR Vγ5/Vδ1 gene without N nucleotide addition (canonical sequence) have been reported to be positively selected in fetal thymus. Because skin intraepithelial lymphocytes (s-IEL) also express canonical sequence, it is proposed that s-IEL are generated by an epidermis- specific homing of these positive selected fetal Vγ5/Vδ1-bearing thymocytes. We have reported that positive selection of Vγ5+ thymocytes is not seen in AKR/J→C3H/He bone marrow chimeras. In this report, we analyzed the phenotype of donor-derived s-IEL from AKR/J→C3H/He bone marrow chimeras that appeared in the absence of positive selection of Vγ5-bearing thymocytes. We found interestingly that almost all donor-derived s-IEL in the bone marrow chimeras expressed TCR-αβ and lack expression of both CD4 and CD8. Furthermore, TCR-αβ+ s-IEL were detected in adult-thymectomized bone marrow chimeras, indicating that the TCR-αβ+ s-IEL are extrathymically derived. All these results suggest that the s-IEL expressing TCR-αβ appear extrathymically in the absence of positive selection of Vγ5/Vδ1-bearing thymocytes. Our results may also explain the contradicting result between human s-IEL, which are TCR-αβ+CD4-CD8- and murine s-IEL, and suggest the importance of extrathymic T cell differentiation pathway of the TCR-αβ lineage in immune surveillance.
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M3 - Article
VL - 151
SP - 3000
EP - 3006
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -