Abstract
Apple (Malus pumila) procyanidins led to a potent vasorelaxation effect μMin 1.0 μM phenylephrine-contractive rat thoracic aorta. Relaxation was greatly reduced by 70 mm KCl as well as by removal of the endothelium, suggesting that it was associated with endothelium- dependent hyperpolarization. Neither cAMP synthesis inhibition nor NAD(P)H oxidase inhibition abolished the effect. In contrast, complete abolition by a soluble guanylyl cyclase inhibitor revealed that apple procya- nidins were mainly involved in the cGMP production pathways. In the presence of NG-monoethyl-l-arginine or tetraethylammonium chloride, the effect was still observed at higher concentrations (>25 μ, g/ml), while their combination completely diminished the effect. Vasorelaxation was to some extent affected by paxillin, apamin and glybenclamide, and was greatly affected by 4-aminopyridine and by BaCl2. These results indicate that procyanidin-induced vasorelaxation is associated with NO-cGMP pathway in combination with hyper- polarization due to multiple activation of Ca2+-dependent and -independent K + channels.
| Original language | English |
|---|---|
| Pages (from-to) | 2246-2251 |
| Number of pages | 6 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 73 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Nov 4 2009 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Apple procyanidins induced vascular relaxation in isolated rat aorta through NO/cGMP pathway in combination with hyperpolarization by multiple K + channel activations. / Matsui, Toshiro; Korematsu, Sachiko; Byun, Eui Baek; Nishizuka, Taichi; Ohshima, Shunji; Kanda, Tomomasa.
In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 10, 04.11.2009, p. 2246-2251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apple procyanidins induced vascular relaxation in isolated rat aorta through NO/cGMP pathway in combination with hyperpolarization by multiple K + channel activations
AU - Matsui, Toshiro
AU - Korematsu, Sachiko
AU - Byun, Eui Baek
AU - Nishizuka, Taichi
AU - Ohshima, Shunji
AU - Kanda, Tomomasa
PY - 2009/11/4
Y1 - 2009/11/4
N2 - Apple (Malus pumila) procyanidins led to a potent vasorelaxation effect μMin 1.0 μM phenylephrine-contractive rat thoracic aorta. Relaxation was greatly reduced by 70 mm KCl as well as by removal of the endothelium, suggesting that it was associated with endothelium- dependent hyperpolarization. Neither cAMP synthesis inhibition nor NAD(P)H oxidase inhibition abolished the effect. In contrast, complete abolition by a soluble guanylyl cyclase inhibitor revealed that apple procya- nidins were mainly involved in the cGMP production pathways. In the presence of NG-monoethyl-l-arginine or tetraethylammonium chloride, the effect was still observed at higher concentrations (>25 μ, g/ml), while their combination completely diminished the effect. Vasorelaxation was to some extent affected by paxillin, apamin and glybenclamide, and was greatly affected by 4-aminopyridine and by BaCl2. These results indicate that procyanidin-induced vasorelaxation is associated with NO-cGMP pathway in combination with hyper- polarization due to multiple activation of Ca2+-dependent and -independent K + channels.
AB - Apple (Malus pumila) procyanidins led to a potent vasorelaxation effect μMin 1.0 μM phenylephrine-contractive rat thoracic aorta. Relaxation was greatly reduced by 70 mm KCl as well as by removal of the endothelium, suggesting that it was associated with endothelium- dependent hyperpolarization. Neither cAMP synthesis inhibition nor NAD(P)H oxidase inhibition abolished the effect. In contrast, complete abolition by a soluble guanylyl cyclase inhibitor revealed that apple procya- nidins were mainly involved in the cGMP production pathways. In the presence of NG-monoethyl-l-arginine or tetraethylammonium chloride, the effect was still observed at higher concentrations (>25 μ, g/ml), while their combination completely diminished the effect. Vasorelaxation was to some extent affected by paxillin, apamin and glybenclamide, and was greatly affected by 4-aminopyridine and by BaCl2. These results indicate that procyanidin-induced vasorelaxation is associated with NO-cGMP pathway in combination with hyper- polarization due to multiple activation of Ca2+-dependent and -independent K + channels.
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UR - http://www.scopus.com/inward/citedby.url?scp=70350451406&partnerID=8YFLogxK
U2 - 10.1271/bbb.90334
DO - 10.1271/bbb.90334
M3 - Article
C2 - 19809179
AN - SCOPUS:70350451406
VL - 73
SP - 2246
EP - 2251
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -