Apple procyanidins induced vascular relaxation in isolated rat aorta through NO/cGMP pathway in combination with hyperpolarization by multiple K + channel activations

Toshiro Matsui, Sachiko Korematsu, Eui Baek Byun, Taichi Nishizuka, Shunji Ohshima, Tomomasa Kanda

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Apple (Malus pumila) procyanidins led to a potent vasorelaxation effect μMin 1.0 μM phenylephrine-contractive rat thoracic aorta. Relaxation was greatly reduced by 70 mm KCl as well as by removal of the endothelium, suggesting that it was associated with endothelium- dependent hyperpolarization. Neither cAMP synthesis inhibition nor NAD(P)H oxidase inhibition abolished the effect. In contrast, complete abolition by a soluble guanylyl cyclase inhibitor revealed that apple procya- nidins were mainly involved in the cGMP production pathways. In the presence of NG-monoethyl-l-arginine or tetraethylammonium chloride, the effect was still observed at higher concentrations (>25 μ, g/ml), while their combination completely diminished the effect. Vasorelaxation was to some extent affected by paxillin, apamin and glybenclamide, and was greatly affected by 4-aminopyridine and by BaCl2. These results indicate that procyanidin-induced vasorelaxation is associated with NO-cGMP pathway in combination with hyper- polarization due to multiple activation of Ca2+-dependent and -independent K + channels.

Original languageEnglish
Pages (from-to)2246-2251
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number10
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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