Application of a quartz crystal microbalance (QCM) twin sensor for selective label-free immunoassay to simultaneous antigen-antibody reactions

Naoki Mizutani, Sergiy Korposh, Roman Selyanchyn, Shunichi Wakamatsu, Seung Woo Lee

Research output: Contribution to specialist publicationArticle

18 Citations (Scopus)

Abstract

In this work a 30 MHz quartz crystal microbalance (QCM) twin sensor system was used for simultaneous monitoring of antigen-antibody reactions. The twin sensor system provided a significant improvement of the conventional mass-sensitive devices, which allows to undertake simultaneous control and analyte measurements under the same experimental conditions. In the given system one channel of the QCM electrode, fixed in a thermostatic flow cell, was modified with chrome pure human IgG (H-IgG) while the second with chrome pure chicken IgY. The antigen-antibody reaction was measured by admitting affinity pure anti-human IgG (Anti-H-IgG) and affinity pure anti-chicken IgG (Anti-C-IgY) into the flow cell and recording the frequency change caused by the selective adsorption of the complementary antigen on the particular antibody immobilized on the surface of the QCM electrode. The system showed high sensitivity with a limit of detection (LOD) of 62.1 ng/mL and fast response time within 5 min.

Original languageEnglish
Pages1-9
Number of pages9
Volume137
No.2
Specialist publicationSensors and Transducers
Publication statusPublished - Feb 1 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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