Application of entrapped growing yeast cells to peptide secretion system

Tsutomu Okada, Kenji Sonomoto, Atsuo Tanaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Efficient production of α-mating factor with immobilized growing Saccharomyces cerevisiae cells was examined in order to establish a secretion system for useful peptides using the α-mating factor secretion system. The free yeast cells produced α-mating factor into the cultured medium, but α-mating factor was gradually degraded in situ. Based on the criteria of α-mating factor productivity, cell growth in gels, cell leakage from gels, and mechanical strength of gels, a mixture of a hydrophilic neutral photo-crosslinkable resin prepolymer (ENT-2000) and an anionic prepolymer (ENTA-2) (8:2 by weight) was employed as gel material, among various kinds of natural and synthetic supports, to entrap the yeast cells. Anionic photo-crosslinkable resins selectively entrapped α-mating factor-degrading enzymes which were produced by the cells and degraded α-mating factor formed. The immobilized growing yeast cells could be used successfully for continuous production of α-mating factor in a packed column reactor for at least 30 days.

Original languageEnglish
Pages (from-to)112-116
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume26
Issue number2
DOIs
Publication statusPublished - May 1 1987
Externally publishedYes

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Mating Factor
Yeasts
Peptides
Gels
Saccharomyces cerevisiae

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Application of entrapped growing yeast cells to peptide secretion system. / Okada, Tsutomu; Sonomoto, Kenji; Tanaka, Atsuo.

In: Applied Microbiology and Biotechnology, Vol. 26, No. 2, 01.05.1987, p. 112-116.

Research output: Contribution to journalArticle

Okada, Tsutomu ; Sonomoto, Kenji ; Tanaka, Atsuo. / Application of entrapped growing yeast cells to peptide secretion system. In: Applied Microbiology and Biotechnology. 1987 ; Vol. 26, No. 2. pp. 112-116.
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