Application of Flow Cytometry for Rapid Determination of Ploidy Levels in Asparagus (Asparagus officinalis L.)

Yukio Ozaki, Kumiko Narikiyo, Michikazu Hiramatsu, Kenji Ureshino, Hiroshi Okubo

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Abstract

Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume43
Issue number1-2
Publication statusPublished - Nov 1998

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All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

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