Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.
|Number of pages||6|
|Journal||Journal of the Faculty of Agriculture, Kyushu University|
|Publication status||Published - Nov 1998|
All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences (miscellaneous)