TY - JOUR
T1 - Application of Flow Cytometry for Rapid Determination of Ploidy Levels in Asparagus (Asparagus officinalis L.)
AU - Ozaki, Yukio
AU - Narikiyo, Kumiko
AU - Hiramatsu, Michikazu
AU - Ureshino, Kenji
AU - Okubo, Hiroshi
PY - 1998/11
Y1 - 1998/11
N2 - Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.
AB - Flow cytometry of PI-stained nuclei was subjected to estimate the ploidy levels in asparagus. No significant difference was observed in PI fluorescent intensity at the prominent peak in diploid cultivars/strain, and there was a very strong positive linear correlation (r=0.9977) between PI fluorescent intensities and ploidy levels in haploid, diploid, triploid and tetraploid cultivars/strains. Flow cytometry has been proven to be a rapid and efficient ploidy analysis during large scale experiments, such as selection of haploid plants from numbers of regenerated plants in anther culture.
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M3 - Article
AN - SCOPUS:0032268156
SN - 0023-6152
VL - 43
SP - 83
EP - 88
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
IS - 1-2
ER -