Application of immobilized lipase to hydrolysis of triacylglyceride

Yoshiharu Kimura; Atsuo Tanaka; Kenji Sonomoto; Takuya Nihira; Saburo Fukui

doi:10.1007/BF00499860

Application of immobilized lipase to hydrolysis of triacylglyceride

Yoshiharu Kimura, Atsuo Tanaka, Kenji Sonomoto, Takuya Nihira, Saburo Fukui

Research output: Contribution to journal › Article › peer-review

130 Citations (Scopus)

Abstract

Lipase from Candida cylindracea was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers, and by covalent binding or by adsorption to different types of porous inorganic or organic supports. All of the immobilized lipase preparations thus obtained showed some activity for hydrolysis of olive oil. Lipase entrapped with a hydrophobic photo-cross-linkable resin prepolymer exhibited the highest activity, which was about 30% of that of the free counterpart. Entrapment method enabled lipase to gain operational stability. Semicontinuous hydrolysis of olive oil using immobilized lipase was also accomplished in a packed-bed reactor with a recycling system. In this reactor, immobilized lipase was observed to have the sufficient activity and stability.

Original language	English
Pages (from-to)	107-112
Number of pages	6
Journal	European Journal of Applied Microbiology and Biotechnology
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/BF00499860
Publication status	Published - Mar 1 1983
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Microbiology

Access to Document

10.1007/BF00499860

Cite this

@article{bb2762e377a1421fba08bdc88005e397,

title = "Application of immobilized lipase to hydrolysis of triacylglyceride",

abstract = "Lipase from Candida cylindracea was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers, and by covalent binding or by adsorption to different types of porous inorganic or organic supports. All of the immobilized lipase preparations thus obtained showed some activity for hydrolysis of olive oil. Lipase entrapped with a hydrophobic photo-cross-linkable resin prepolymer exhibited the highest activity, which was about 30% of that of the free counterpart. Entrapment method enabled lipase to gain operational stability. Semicontinuous hydrolysis of olive oil using immobilized lipase was also accomplished in a packed-bed reactor with a recycling system. In this reactor, immobilized lipase was observed to have the sufficient activity and stability.",

author = "Yoshiharu Kimura and Atsuo Tanaka and Kenji Sonomoto and Takuya Nihira and Saburo Fukui",

year = "1983",

month = mar,

day = "1",

doi = "10.1007/BF00499860",

language = "English",

volume = "17",

pages = "107--112",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer Verlag",

number = "2",

}

TY - JOUR

T1 - Application of immobilized lipase to hydrolysis of triacylglyceride

AU - Kimura, Yoshiharu

AU - Tanaka, Atsuo

AU - Sonomoto, Kenji

AU - Nihira, Takuya

AU - Fukui, Saburo

PY - 1983/3/1

Y1 - 1983/3/1

N2 - Lipase from Candida cylindracea was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers, and by covalent binding or by adsorption to different types of porous inorganic or organic supports. All of the immobilized lipase preparations thus obtained showed some activity for hydrolysis of olive oil. Lipase entrapped with a hydrophobic photo-cross-linkable resin prepolymer exhibited the highest activity, which was about 30% of that of the free counterpart. Entrapment method enabled lipase to gain operational stability. Semicontinuous hydrolysis of olive oil using immobilized lipase was also accomplished in a packed-bed reactor with a recycling system. In this reactor, immobilized lipase was observed to have the sufficient activity and stability.

AB - Lipase from Candida cylindracea was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers, and by covalent binding or by adsorption to different types of porous inorganic or organic supports. All of the immobilized lipase preparations thus obtained showed some activity for hydrolysis of olive oil. Lipase entrapped with a hydrophobic photo-cross-linkable resin prepolymer exhibited the highest activity, which was about 30% of that of the free counterpart. Entrapment method enabled lipase to gain operational stability. Semicontinuous hydrolysis of olive oil using immobilized lipase was also accomplished in a packed-bed reactor with a recycling system. In this reactor, immobilized lipase was observed to have the sufficient activity and stability.

UR - http://www.scopus.com/inward/record.url?scp=0020608847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020608847&partnerID=8YFLogxK

U2 - 10.1007/BF00499860

DO - 10.1007/BF00499860

M3 - Article

AN - SCOPUS:0020608847

SN - 0175-7598

VL - 17

SP - 107

EP - 112

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 2

ER -

Application of immobilized lipase to hydrolysis of triacylglyceride

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this