Application of immobilized lipase to regio-specific interesterification of triglyceride in organic solvent

Kenzo Yokozeki, Shigeru Yamanaka, Koichi Takinami, Yoshio Hirose, Atsuo Tanaka, Kenji Sonomoto, Saburo Fukui

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Lipase from Rhizopus delemar was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers or by binding to various types of porous silica beads. The immobilized lipase preparations thus obtained were examined for their activity in converting olive oil to an interesterified fat (cacao butter-like fat), whose oleic acid moieties at 1- and 3-positions were replaced with stearic acid moieties, in the reaction solvent n-hexane. Although all of the immobilized preparations exhibited some activity, lipase adsorbed on Celite and then entrapped with a hydrophobic photo-crosslinkable resin prepolymer showed the highest activity, about 75% of that of lipase simply adsorbed onto Celite. Entrapment markedly enhanced the operational stability of lipase.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalEuropean Journal of Applied Microbiology and Biotechnology
Issue number1
Publication statusPublished - Mar 1 1982


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology

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