TY - JOUR
T1 - Application of micronucleus in vitro assay to micropollutants in river water
AU - Chung, Youn Son
AU - Ichikawa, Kazuhiro
AU - Utsumi, Hideo
N1 - Funding Information:
This work was in part supported by a Grant-in-Aid for Scientific Research and that for Research on Priority Areas for Cooperative Research. for General Science Research. and for Developmental Scientific Research from the Ministry of Education. Science. Sports and Culture of Japan. by Naito Foundation, and by the Cosmetology Research Foundation and a Grant from Tokyo Foundation for Better Environment.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - To determine the genotoxicity of river water towards mammalian cells, we applied Micronucleus in vitro test using mammalian cells to the samples taken from river Tamagawa located between Tokyo and Kanagawa prefecture. Water samples were condensed by Sep-pak cartridges and extracted by dichloromethane and methanol. Positive genotoxicity was observed in methanol extracts from sampling stations of Hinobashi and Marukobashi, while no dichloromethane extracts showed genotoxicity, suggesting that polar genotoxic micropollutants may be contained in the water of Tamagawa, at least in its down-stream. Significantly high mutagenicity also detected from Hinobashi and Marukobashi by Ames mutagenicity test using Salmonella Typhimurium, and some difference was obtained in sensitivity between the two methods. This may arise from the difference in species used, that is, mammalian cells in micronucleus test and bacteria in Ames mutagenicity test.
AB - To determine the genotoxicity of river water towards mammalian cells, we applied Micronucleus in vitro test using mammalian cells to the samples taken from river Tamagawa located between Tokyo and Kanagawa prefecture. Water samples were condensed by Sep-pak cartridges and extracted by dichloromethane and methanol. Positive genotoxicity was observed in methanol extracts from sampling stations of Hinobashi and Marukobashi, while no dichloromethane extracts showed genotoxicity, suggesting that polar genotoxic micropollutants may be contained in the water of Tamagawa, at least in its down-stream. Significantly high mutagenicity also detected from Hinobashi and Marukobashi by Ames mutagenicity test using Salmonella Typhimurium, and some difference was obtained in sensitivity between the two methods. This may arise from the difference in species used, that is, mammalian cells in micronucleus test and bacteria in Ames mutagenicity test.
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U2 - 10.1016/S0273-1223(97)00145-5
DO - 10.1016/S0273-1223(97)00145-5
M3 - Conference article
AN - SCOPUS:0030840902
VL - 35
SP - 9
EP - 13
JO - Water Science and Technology
JF - Water Science and Technology
SN - 0273-1223
IS - 8
T2 - Proceedings of the 1995 5th IAWQ Asian Regional Conference on Water Quality and Pollution Control
Y2 - 7 February 1995 through 9 February 1995
ER -