Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.

Tomoya Ishii, Kentaro Muraki, Naohiko Shimada, Arihiro Kano, Nao Nishida, Katsushi Tokunaga, Atsushi Maruyama

Research output: Contribution to journalArticle

Abstract

We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.

Original languageEnglish
Pages (from-to)237-238
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number52
Publication statusPublished - Dec 1 2008

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Ishii, T., Muraki, K., Shimada, N., Kano, A., Nishida, N., Tokunaga, K., & Maruyama, A. (2008). Application of partially double-stranded DNA probes to high-throughput SNPs genotyping. Nucleic acids symposium series (2004), (52), 237-238.