Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.

Tomoya Ishii, Kentaro Muraki, Naohiko Shimada, Arihiro Kano, Nao Nishida, Katsushi Tokunaga, Atsushi Maruyama

Research output: Contribution to journalArticle

Abstract

We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.

Original languageEnglish
Pages (from-to)237-238
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number52
Publication statusPublished - Dec 1 2008

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DNA Probes
Single Nucleotide Polymorphism
DNA
Ligases
Oligonucleotide Array Sequence Analysis
Technology

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Ishii, T., Muraki, K., Shimada, N., Kano, A., Nishida, N., Tokunaga, K., & Maruyama, A. (2008). Application of partially double-stranded DNA probes to high-throughput SNPs genotyping. Nucleic acids symposium series (2004), (52), 237-238.

Application of partially double-stranded DNA probes to high-throughput SNPs genotyping. / Ishii, Tomoya; Muraki, Kentaro; Shimada, Naohiko; Kano, Arihiro; Nishida, Nao; Tokunaga, Katsushi; Maruyama, Atsushi.

In: Nucleic acids symposium series (2004), No. 52, 01.12.2008, p. 237-238.

Research output: Contribution to journalArticle

Ishii, T, Muraki, K, Shimada, N, Kano, A, Nishida, N, Tokunaga, K & Maruyama, A 2008, 'Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.', Nucleic acids symposium series (2004), no. 52, pp. 237-238.
Ishii, Tomoya ; Muraki, Kentaro ; Shimada, Naohiko ; Kano, Arihiro ; Nishida, Nao ; Tokunaga, Katsushi ; Maruyama, Atsushi. / Application of partially double-stranded DNA probes to high-throughput SNPs genotyping. In: Nucleic acids symposium series (2004). 2008 ; No. 52. pp. 237-238.
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