Application of photo-crosslinkable resin prepolymers to entrap microbial cells. Effects of increased cell-entrapping gel hydrophobicity on the hydrocortisone Δ1-Dehydrogenation

K. Sonomoto, A. Tanaka, T. Omata, T. Yamane, S. Fukui

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Acetone-dried cells of Arthrobacter simplex, whose steroid Δ1 activity had been previously induced, were entrapped by the use of photo-crosslinkable resin prepolymers. When the hydrophobicity of the cell-entrapping gel was increased by mixing a hydrophobic prepolymer (main chain component; polypropyleneglycol) with a hydrophilic prepolymer (main chain component; polypropyleneglycol) with a hydrophilic prepolymer (main chain component; polyethyleneglycol) (up to 30%), the hydrocortisone to prednisolone conversion rate of the immobilized cells increased significantly, attaining approximately 20% of that of the free cells. A 10% addition of organic solvents, such as methanol, to the aqueous reaction mixture enhanced the solubility of the substrate greatly and to a lesser degree the reaction rate of the immobilized cells. The presence of an electron acceptor, phenazine methosulfate or 2,6-dichlorophenolindophenol, stimulated the steroid conversion of the entrapped as well as the free cells. The stability of the entrapped cells over repeated reactions was improved by immobilization.

Original languageEnglish
Pages (from-to)325-334
Number of pages10
JournalEuropean Journal of Applied Microbiology and Biotechnology
Volume6
Issue number4
DOIs
Publication statusPublished - Dec 1979

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology

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