Application of Pluronic F68 for Aqueous Two-Phase Extraction of Proteins

Toshihiko Kitahara, Masamichi Kamihira, Hiroshi Takeuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Studies were made of the application of a block copolymer of polyethylene oxide and polypropylene oxide, Pluronic F68, to aqueous two-phase extraction of proteins. For Pluronic F68/salt systems, the effects of temperature, salt concentration and pH on the binodials and protein partitioning were studied. Pluronic F68/ salt system had a region in which Pluronic F68 becomes solid in phase diagram at 25 °C. Partitioning of the proteins between the two phases could be controlled by varying salt composition. Further applicability of the Pluronic F68/ammonium sulfate system was examined for the purification of monoclonal antibodies from various hybridoma culture broths. Aqueous two-phase extraction provided a simple and rapid separation and concentration method with high recovery.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume26
Issue number2
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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