Application of Pluronic F68 for Aqueous Two-Phase Extraction of Proteins

Toshihiko Kitahara, Masamichi Kamihira, Hiroshi Takeuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Studies were made of the application of a block copolymer of polyethylene oxide and polypropylene oxide, Pluronic F68, to aqueous two-phase extraction of proteins. For Pluronic F68/salt systems, the effects of temperature, salt concentration and pH on the binodials and protein partitioning were studied. Pluronic F68/ salt system had a region in which Pluronic F68 becomes solid in phase diagram at 25 °C. Partitioning of the proteins between the two phases could be controlled by varying salt composition. Further applicability of the Pluronic F68/ammonium sulfate system was examined for the purification of monoclonal antibodies from various hybridoma culture broths. Aqueous two-phase extraction provided a simple and rapid separation and concentration method with high recovery.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalJournal of Chemical Engineering of Japan
Volume26
Issue number2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Poloxamer
Salts
Proteins
Polypropylene oxides
Monoclonal antibodies
Polyethylene oxides
Block copolymers
Phase diagrams
Purification
Ammonium Sulfate
Recovery
Monoclonal Antibodies
Chemical analysis
Temperature

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Application of Pluronic F68 for Aqueous Two-Phase Extraction of Proteins. / Kitahara, Toshihiko; Kamihira, Masamichi; Takeuchi, Hiroshi.

In: Journal of Chemical Engineering of Japan, Vol. 26, No. 2, 1993, p. 183-188.

Research output: Contribution to journalArticle

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