Application of polymer-protected ultrafine platinum particles to the immunological detection of human serum albumin

Taizo Uda, Rie Kanmei, Shigeru Akasofu, Emi Hifumi, Michitaka Ohtaki

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multiepitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 110 μg/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.

    Original languageEnglish
    Pages (from-to)259-264
    Number of pages6
    JournalAnalytical Biochemistry
    Volume218
    Issue number2
    DOIs
    Publication statusPublished - May 1 1994

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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