Application of polymer-protected ultrafine platinum particles to the immunological detection of human serum albumin

Taizo Uda, Rie Kanmei, Shigeru Akasofu, Emi Hifumi, Michitaka Ohtaki

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multiepitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 110 μg/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.

    Original languageEnglish
    Pages (from-to)259-264
    Number of pages6
    JournalAnalytical Biochemistry
    Volume218
    Issue number2
    DOIs
    Publication statusPublished - May 1 1994

    Fingerprint

    Platinum
    Serum Albumin
    Polymers
    Immunochemistry
    Molecules
    Ultrafine
    Limit of Detection
    Assays
    Proteins
    Enzyme-Linked Immunosorbent Assay
    Monoclonal Antibodies
    Antigens
    Antibodies

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Application of polymer-protected ultrafine platinum particles to the immunological detection of human serum albumin. / Uda, Taizo; Kanmei, Rie; Akasofu, Shigeru; Hifumi, Emi; Ohtaki, Michitaka.

    In: Analytical Biochemistry, Vol. 218, No. 2, 01.05.1994, p. 259-264.

    Research output: Contribution to journalArticle

    @article{2e506f2154e04663857a2371355a0a6f,
    title = "Application of polymer-protected ultrafine platinum particles to the immunological detection of human serum albumin",
    abstract = "The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multiepitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 110 μg/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.",
    author = "Taizo Uda and Rie Kanmei and Shigeru Akasofu and Emi Hifumi and Michitaka Ohtaki",
    year = "1994",
    month = "5",
    day = "1",
    doi = "10.1006/abio.1994.1176",
    language = "English",
    volume = "218",
    pages = "259--264",
    journal = "Analytical Biochemistry",
    issn = "0003-2697",
    publisher = "Academic Press Inc.",
    number = "2",

    }

    TY - JOUR

    T1 - Application of polymer-protected ultrafine platinum particles to the immunological detection of human serum albumin

    AU - Uda, Taizo

    AU - Kanmei, Rie

    AU - Akasofu, Shigeru

    AU - Hifumi, Emi

    AU - Ohtaki, Michitaka

    PY - 1994/5/1

    Y1 - 1994/5/1

    N2 - The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multiepitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 110 μg/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.

    AB - The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multiepitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 110 μg/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.

    UR - http://www.scopus.com/inward/record.url?scp=0028208677&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0028208677&partnerID=8YFLogxK

    U2 - 10.1006/abio.1994.1176

    DO - 10.1006/abio.1994.1176

    M3 - Article

    C2 - 8074278

    AN - SCOPUS:0028208677

    VL - 218

    SP - 259

    EP - 264

    JO - Analytical Biochemistry

    JF - Analytical Biochemistry

    SN - 0003-2697

    IS - 2

    ER -