Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean

Jae Won Lee, Takato Uchikata, Atsuki Matsubara, Takuji Nakamura, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A metabolomics technology for lipid profiling based on supercritical fluid chromatography (SFC) coupled with mass spectrometry (MS) was applied to analyze lipids of soybean. Principal component analysis (PCA) was used to discriminate twelve soybean cultivars according to their suitability for different processed foods such as natto, tofu, edamame, and nimame. By PCA assay, triacylglycerol (TAG) was found as the main variable for discrimination of soybean cultivars. Therefore, a high-throughput and high-resolution TAG profiling method by SFC/MS was developed to more effective discrimination. By investigating several columns, three Chromolith Performance RP-18e columns connected in series were chosen as the most effective column for TAG profiling. Diverse TAGs were separated effectively for 8. min without purification. Additionally, each TAG was identified successfully by the programmed cone voltage fragmentation even without MS/MS analysis and any standard sample.

Original languageEnglish
Pages (from-to)262-268
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

Fingerprint

Supercritical Fluid Chromatography
Soybeans
Lipids
Mass spectrometry
Mass Spectrometry
Triglycerides
Soy Foods
Principal Component Analysis
Principal component analysis
Processed foods
Metabolomics
Tandem Mass Spectrometry
Purification
Cones
Assays
Throughput
Technology
Food
Electric potential

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean. / Lee, Jae Won; Uchikata, Takato; Matsubara, Atsuki; Nakamura, Takuji; Fukusaki, Eiichiro; Bamba, Takeshi.

In: Journal of Bioscience and Bioengineering, Vol. 113, No. 2, 01.02.2012, p. 262-268.

Research output: Contribution to journalArticle

Lee, Jae Won ; Uchikata, Takato ; Matsubara, Atsuki ; Nakamura, Takuji ; Fukusaki, Eiichiro ; Bamba, Takeshi. / Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean. In: Journal of Bioscience and Bioengineering. 2012 ; Vol. 113, No. 2. pp. 262-268.
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