Application of Urethane Prepolymers to Immobilization of Biocatalysts: Δ1-Dehydrogenation of Hydrocortisone by Arthrobacter simplex Cells Entrapped with Urethane Prepolymers

Kenji Sonomoto, Atsuo Tanaka, Saburo Fukui, Ing Nyol Jin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Acetone-dried cells of Arthrobacter simplex having appreciable steroid Δ1-dehydrogenase activity were immobilized by mixing the cell suspension with water-miscible urethane prepolymers synthesized from toluene diisocyanate and polyether diols. The entrapped cell activity in the transformation of hydrocortisone to prednisolone was affected by the properties of urethane prepolymers, such as the isocyanate group content in prepolymers, the molecular weight of polyether diols and the ethylene oxide content in diols. The addition of 10% of organic solvents, such as methanol and glycols, to the aqueous reaction mixture enhanced the solubility of the substrate greatly and the reaction rate of the immobilized cells. The activity of immobilized cells remained high even in the system containing 30% of methanol, which drastically inhibited the activity of free cells. The presence of an electron acceptor, phenazine methosulfate or 2, 6-dichlorophenolindophenol, significantly stimulated the steroid conversion with entrapped cells, as well as free cells. The stability of the cells over repeated reactions was greatly improved by immobilization.

Original languageEnglish
Pages (from-to)1119-1126
Number of pages8
JournalAgricultural and Biological Chemistry
Volume44
Issue number5
DOIs
Publication statusPublished - Jan 1 1980

Fingerprint

Pimelobacter simplex
Arthrobacter
urethane
Urethane
Dehydrogenation
Immobilization
cortisol
Hydrocortisone
glycols
Cells
Immobilized Cells
Polyethers
3-oxosteroid delta(1) dehydrogenase
Enzymes
Methanol
immobilized cells
Toluene 2,4-Diisocyanate
Methylphenazonium Methosulfate
2,6-Dichloroindophenol
cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Application of Urethane Prepolymers to Immobilization of Biocatalysts : Δ1-Dehydrogenation of Hydrocortisone by Arthrobacter simplex Cells Entrapped with Urethane Prepolymers. / Sonomoto, Kenji; Tanaka, Atsuo; Fukui, Saburo; Jin, Ing Nyol.

In: Agricultural and Biological Chemistry, Vol. 44, No. 5, 01.01.1980, p. 1119-1126.

Research output: Contribution to journalArticle

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