Application of whole mouse embryo culture system on stem cell research

Kasem Kulkeaw; Chiyo Mizuochi; Yuka Horio; Noriko Osumi; Kohichiro Tsuji; Daisuke Sugiyama

doi:10.1007/s12015-009-9064-2

Application of whole mouse embryo culture system on stem cell research

Kasem Kulkeaw, Chiyo Mizuochi, Yuka Horio, Noriko Osumi, Kohichiro Tsuji, Daisuke Sugiyama

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O₂ and CO₂. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Stem Cell Reviews and Reports
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/s12015-009-9064-2
Publication status	Published - Jun 2009

All Science Journal Classification (ASJC) codes

Cell Biology
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12015-009-9064-2

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title = "Application of whole mouse embryo culture system on stem cell research",

abstract = "The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O2 and CO2. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.",

author = "Kasem Kulkeaw and Chiyo Mizuochi and Yuka Horio and Noriko Osumi and Kohichiro Tsuji and Daisuke Sugiyama",

note = "Funding Information: Acknowledgements The authors would like to thank our grant supports from Special Coordination Funds for Promoting Science and Technology, Midori Nakagawa and Tatsuya Sasaki for their sincere supports in our laboratory and Drs. Tani Kenzaburo and Koichi Akashi for helpful discussion. Kasem Kulkeaw is a recipient of Ajinomoto scholarship foundation.",

year = "2009",

month = jun,

doi = "10.1007/s12015-009-9064-2",

language = "English",

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pages = "175--180",

journal = "Stem Cell Reviews",

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T1 - Application of whole mouse embryo culture system on stem cell research

AU - Kulkeaw, Kasem

AU - Mizuochi, Chiyo

AU - Horio, Yuka

AU - Osumi, Noriko

AU - Tsuji, Kohichiro

AU - Sugiyama, Daisuke

N1 - Funding Information: Acknowledgements The authors would like to thank our grant supports from Special Coordination Funds for Promoting Science and Technology, Midori Nakagawa and Tatsuya Sasaki for their sincere supports in our laboratory and Drs. Tani Kenzaburo and Koichi Akashi for helpful discussion. Kasem Kulkeaw is a recipient of Ajinomoto scholarship foundation.

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Y1 - 2009/6

N2 - The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O2 and CO2. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.

AB - The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O2 and CO2. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.

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Application of whole mouse embryo culture system on stem cell research

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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