Application of whole mouse embryo culture system on stem cell research

Kasem Kulkeaw, Chiyo Mizuochi, Yuka Horio, Noriko Osumi, Kohichiro Tsuji, Daisuke Sugiyama

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O2 and CO2. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.

    Original languageEnglish
    Pages (from-to)175-180
    Number of pages6
    JournalStem Cell Reviews and Reports
    Volume5
    Issue number2
    DOIs
    Publication statusPublished - Jun 2009

    All Science Journal Classification (ASJC) codes

    • Cell Biology
    • Cancer Research

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