TY - JOUR
T1 - Application to Photocatalytic H2Production of a Whole-Cell Reaction by Recombinant Escherichia coli Cells Expressing [FeFe]-Hydrogenase and Maturases Genes
AU - Honda, Yuki
AU - Hagiwara, Hidehisa
AU - Ida, Shintaro
AU - Ishihara, Tatsumi
N1 - Funding Information:
We thank Dr. Toshihisa Ohshima and Dr. Toshihide Okajima for their advice on protein manipulation under anaerobic conditions. This work was supported by the International Institute for Carbon-Neutral Energy Research (WPI-I
Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2016/7/4
Y1 - 2016/7/4
N2 - A photocatalytic H2production system using an inorganic–bio hybrid photocatalyst could contribute to the efficient utilization of solar energy, but would require the development of a new approach for preparing a H2-forming biocatalyst. In the present study, we constructed a recombinant strain of Escherichia coli expressing the genes encoding the [FeFe]-hydrogenase and relevant maturases from Clostridium acetobutylicum NBRC 13948 for use as a biocatalyst. We investigated the direct application of a whole-cell of the recombinant E. coli. The combination of TiO2, methylviologen, and the recombinant E. coli formed H2under light irradiation, demonstrating that whole cells of the recombinant E. coli could be employed for photocatalytic H2production without any time-consuming and costly manipulations (for example, enzyme purification). This is the first report of the direct application of a whole-cell reaction of recombinant E. coli to photocatalytic H2production.
AB - A photocatalytic H2production system using an inorganic–bio hybrid photocatalyst could contribute to the efficient utilization of solar energy, but would require the development of a new approach for preparing a H2-forming biocatalyst. In the present study, we constructed a recombinant strain of Escherichia coli expressing the genes encoding the [FeFe]-hydrogenase and relevant maturases from Clostridium acetobutylicum NBRC 13948 for use as a biocatalyst. We investigated the direct application of a whole-cell of the recombinant E. coli. The combination of TiO2, methylviologen, and the recombinant E. coli formed H2under light irradiation, demonstrating that whole cells of the recombinant E. coli could be employed for photocatalytic H2production without any time-consuming and costly manipulations (for example, enzyme purification). This is the first report of the direct application of a whole-cell reaction of recombinant E. coli to photocatalytic H2production.
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U2 - 10.1002/anie.201600177
DO - 10.1002/anie.201600177
M3 - Article
AN - SCOPUS:84971474759
SN - 1433-7851
VL - 55
SP - 8045
EP - 8048
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 28
ER -