Applying a PUF/hepatocyte spheroid packed-bed type artificial liver to a drug induced hepatic failure rat. - Improvement of the medical treatment effect by optimization of the system -

Hiroyuki Ijima, S. Wada, K. Nakazawa, T. Matsushita, K. Funatsu

Research output: Contribution to journalArticle

Abstract

We developed a hybrid artificial liver (Type 1, module volume: 5.65 cm3) utilizing PUF/hepatocyte spheroid culture, 80% of hepatic failure rats which induced by intraperitoneal injection of 0.5 g/kg-rat D-galactosamine could be recovered by extracorporeal circulation including this module. Then, optimization of the system was tried to aim at recovering a severed hepatic failure rat. A module volume was scaling up from 5.65 cm3 to 18.8 cm3 at 3.3 times (Type 2) and an extracorporeal blood circulation flow rate and a module side plasma circulation flow rate were changed from 0.5 to 1.0 ml/min and from 3.0 to 45.0 ml/min, respectively. In the results, blood ammonia of the hepatic failure rats which induced by 0.75 g/kg rat D-galactosamine (PT=35.9 sec, normal value=20 sec) were well metabolized and four rats were recovered out of five by using a Type 2 module (recovery ratio is 80%). But the rat was died by using a Type I module. On the contrary, blood ammonia concentration was dramatically increased in the control experiments, and three rats were died out of four by using a Type 2 module (recovery ratio is 25%). From the above, it seems that the optimized artificial liver support system is effective for recovering the severer hepatic failure rats.

Original languageEnglish
Pages (from-to)719-723
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume26
Issue number3
Publication statusPublished - 1997

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Artificial Liver
Liver Failure
Hepatocytes
Pharmaceutical Preparations
Galactosamine
Therapeutics
Extracorporeal Circulation
Ammonia
Blood Circulation
Intraperitoneal Injections
Reference Values

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

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title = "Applying a PUF/hepatocyte spheroid packed-bed type artificial liver to a drug induced hepatic failure rat. - Improvement of the medical treatment effect by optimization of the system -",
abstract = "We developed a hybrid artificial liver (Type 1, module volume: 5.65 cm3) utilizing PUF/hepatocyte spheroid culture, 80{\%} of hepatic failure rats which induced by intraperitoneal injection of 0.5 g/kg-rat D-galactosamine could be recovered by extracorporeal circulation including this module. Then, optimization of the system was tried to aim at recovering a severed hepatic failure rat. A module volume was scaling up from 5.65 cm3 to 18.8 cm3 at 3.3 times (Type 2) and an extracorporeal blood circulation flow rate and a module side plasma circulation flow rate were changed from 0.5 to 1.0 ml/min and from 3.0 to 45.0 ml/min, respectively. In the results, blood ammonia of the hepatic failure rats which induced by 0.75 g/kg rat D-galactosamine (PT=35.9 sec, normal value=20 sec) were well metabolized and four rats were recovered out of five by using a Type 2 module (recovery ratio is 80{\%}). But the rat was died by using a Type I module. On the contrary, blood ammonia concentration was dramatically increased in the control experiments, and three rats were died out of four by using a Type 2 module (recovery ratio is 25{\%}). From the above, it seems that the optimized artificial liver support system is effective for recovering the severer hepatic failure rats.",
author = "Hiroyuki Ijima and S. Wada and K. Nakazawa and T. Matsushita and K. Funatsu",
year = "1997",
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AU - Matsushita, T.

AU - Funatsu, K.

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