酵母の凍結ストレス耐性改善へのアプローチ (セミナー 低温/乾燥に適応した生物の生き残り戦略としての休眠)

Translated title of the contribution: Approaches of Improvement of Freezing-Stress Tolerance of Yeast

本城 賢一, 町田 豪, 菅 向志郎, 宮本 敬久

Research output: Contribution to journalArticle

Abstract

Freezing is one of extreme environments for organisms. On the other hand, freezing is useful strategy for storage of foods. The authors have studied on development of freezing tolerance of plants for storage and stable supply of foods. In order to understand the mechanisms of the freezing tolerance, low-temperature inducible genes were isolated from Chlorella, and genes responsible for synthesis of taurine and trehalose were isolated from common carp and tobacco plant, respectively. The cloned (LEA, FAD2, NTRC, PRX, CDO, CSD, TPS, TPP) genes were modified and respectively subcloned into expression vectors for expression in Saccharomyces cerevisiae. Freezing tolerance of the yeast cells expressing the genes was investigated to estimate functions of the expressed gene products.
Original languageJapanese
Pages (from-to)13-17
Number of pages5
Journal低温生物工学会誌 = Cryobiology and cryotechnology
Volume61
Issue number1
Publication statusPublished - 2015

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stress tolerance
freezing
yeasts
cold tolerance
genes
food storage
taurine
Chlorella
trehalose
Cyprinus carpio
Saccharomyces cerevisiae
tobacco
synthesis
organisms
temperature
cells

Cite this

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title = "酵母の凍結ストレス耐性改善へのアプローチ (セミナー 低温/乾燥に適応した生物の生き残り戦略としての休眠)",
abstract = "Freezing is one of extreme environments for organisms. On the other hand, freezing is useful strategy for storage of foods. The authors have studied on development of freezing tolerance of plants for storage and stable supply of foods. In order to understand the mechanisms of the freezing tolerance, low-temperature inducible genes were isolated from Chlorella, and genes responsible for synthesis of taurine and trehalose were isolated from common carp and tobacco plant, respectively. The cloned (LEA, FAD2, NTRC, PRX, CDO, CSD, TPS, TPP) genes were modified and respectively subcloned into expression vectors for expression in Saccharomyces cerevisiae. Freezing tolerance of the yeast cells expressing the genes was investigated to estimate functions of the expressed gene products.",
author = "賢一 本城 and 豪 町田 and 向志郎 菅 and 敬久 宮本",
year = "2015",
language = "Japanese",
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AU - 本城, 賢一

AU - 町田, 豪

AU - 菅, 向志郎

AU - 宮本, 敬久

PY - 2015

Y1 - 2015

N2 - Freezing is one of extreme environments for organisms. On the other hand, freezing is useful strategy for storage of foods. The authors have studied on development of freezing tolerance of plants for storage and stable supply of foods. In order to understand the mechanisms of the freezing tolerance, low-temperature inducible genes were isolated from Chlorella, and genes responsible for synthesis of taurine and trehalose were isolated from common carp and tobacco plant, respectively. The cloned (LEA, FAD2, NTRC, PRX, CDO, CSD, TPS, TPP) genes were modified and respectively subcloned into expression vectors for expression in Saccharomyces cerevisiae. Freezing tolerance of the yeast cells expressing the genes was investigated to estimate functions of the expressed gene products.

AB - Freezing is one of extreme environments for organisms. On the other hand, freezing is useful strategy for storage of foods. The authors have studied on development of freezing tolerance of plants for storage and stable supply of foods. In order to understand the mechanisms of the freezing tolerance, low-temperature inducible genes were isolated from Chlorella, and genes responsible for synthesis of taurine and trehalose were isolated from common carp and tobacco plant, respectively. The cloned (LEA, FAD2, NTRC, PRX, CDO, CSD, TPS, TPP) genes were modified and respectively subcloned into expression vectors for expression in Saccharomyces cerevisiae. Freezing tolerance of the yeast cells expressing the genes was investigated to estimate functions of the expressed gene products.

M3 - 記事

VL - 61

SP - 13

EP - 17

JO - Cryobiology and cryotechnology

JF - Cryobiology and cryotechnology

SN - 1340-7902

IS - 1

ER -