Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen

Yoshihiro Izumi, Shimpei Aikawa, Fumio Matsuda, Tomohisa Hasunuma, Akihiko Kondo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cyanobacterial glycogen has gained interest as a valuable biomass feedstock for biofuel production. However, an ideal method for native glycogen quantification has not been developed. Here, we have proposed a simple methodology that enables the quantitative determination of cyanobacterial glycogen concentration with high repeatability using aqueous size-exclusion chromatography with a differential refractive index detector (SEC/RID). Our SEC/RID system also allows size distributions for native glycogen based on hydrodynamic volumes (Vh), which is proportional to the product of the molecular mass (M) and intrinsic viscosity [η], obtained by universal calibration using linear homopolymers of known M with Mark-Houwink-Sakurada parameters. The universal calibration curve achieved a broad linear range (Vh parameter [η]M=2×102-8×108mLg-1) with a high correlation coefficient (R2=0.9942), because the developed system is equipped with an OHpak SB-806M HQ aqueous column containing four types of polyhydroxy methacrylate-based particles with different particle and pore sizes. Based on the SEC/RID system, response of molecular size distribution of glycogen in microalgae to the cultivation condition was first observed. Our established SEC/RID method has several advantages over conventional techniques, including the simultaneous quantitative and size distribution analyses of glycogen, and represents a potentially useful tool to elucidate the relationship between structural properties and the roles of glycogen in metabolism.

Original languageEnglish
Pages (from-to)90-97
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume930
DOIs
Publication statusPublished - Jul 1 2013
Externally publishedYes

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Glycogen
Refractometry
Size exclusion chromatography
Gel Chromatography
Refractive index
Detectors
Calibration
Microalgae
Biofuels
Methacrylates
Molecular mass
Hydrodynamics
Homopolymerization
Particle Size
Metabolism
Viscosity
Biomass
Feedstocks
Pore size
Structural properties

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen. / Izumi, Yoshihiro; Aikawa, Shimpei; Matsuda, Fumio; Hasunuma, Tomohisa; Kondo, Akihiko.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 930, 01.07.2013, p. 90-97.

Research output: Contribution to journalArticle

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