Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism

Akiko Maruyama-Nakashita, Yumiko Nakamura, Takayuki Tohge, Kazuki Saito, Hideki Takahashi

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Sulfur is an essential macronutrient required for plant growth. To identify key transcription factors regulating the sulfur assimilatory pathway, we screened Arabidopsis thaliana mutants using a fluorescent reporter gene construct consisting of the sulfur limitation-responsive promoter of the SULTR1;2 sulfate transporter and green fluorescent protein as a background indicator for monitoring plant sulfur responses. The isolated mutant, sulfur limitation1 (slim1), was unable to induce SULTR1;2 transcripts under low-sulfur (-S) conditions. Mutations causing the sulfur limitation responseless phenotypes of slim1 were identified in an EIL family transcription factor, ETHYLENE-INSENSITIVE3-LIKE3 (EIL3), whose functional identity with SLIM1 was confirmed by genetic complementation. Sulfate uptake and plant growth on -S were significantly reduced by slim1 mutations but recovered by overexpression of SLIM1. SLIM1 functioned as a central transcriptional regulator, which controlled both the activation of sulfate acquisition and degradation of glucosinolates under-S conditions. Metabolite analysis indicated stable accumulation of glucosinolates in slim1 mutants, even under -S conditions, particularly in the molecular species with methylsulfinylalkyl side chains beneficial to human health. Overexpression of SLIM1 and its rice (Oryza sativa) homologs, but no other EIL genes of Arabidopsis, restored the sulfur limitation responseless phenotypes of slim1 mutants, suggesting uniqueness of the SLIM1/EIL3 subgroup members as sulfur response regulators.

Original languageEnglish
Pages (from-to)3235-3251
Number of pages17
JournalPlant Cell
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 1 2006
Externally publishedYes

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Sulfur
Arabidopsis
Metabolism
sulfur
transcription factors
metabolism
Sulfates
Glucosinolates
mutants
sulfates
glucosinolates
ethylene
Transcription Factors
Genes
plant growth
genetic complementation
Phenotype
mutation
phenotype
Mutation

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. / Maruyama-Nakashita, Akiko; Nakamura, Yumiko; Tohge, Takayuki; Saito, Kazuki; Takahashi, Hideki.

In: Plant Cell, Vol. 18, No. 11, 01.11.2006, p. 3235-3251.

Research output: Contribution to journalArticle

Maruyama-Nakashita, Akiko ; Nakamura, Yumiko ; Tohge, Takayuki ; Saito, Kazuki ; Takahashi, Hideki. / Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. In: Plant Cell. 2006 ; Vol. 18, No. 11. pp. 3235-3251.
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