Archaeal diversity and distribution along thermal and geochemical gradients in hydrothermal sediments at the yonaguni knoll IV hydrothermal field in the Southern Okinawa Trough

Takuro Nunoura, Hanako Oida, Miwako Nakaseama, Ayako Kosaka, Satoru B. Ohkubo, Toru Kikuchi, Hiromi Kazama, Shoko Hosoi-Tanabe, Ko Ichi Nakamura, Masataka Kinoshita, Hisako Hirayama, Fumio Inagaki, Urumu Tsunogai, Jun Ichiro Ishibashi, Ken Takai

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Abstract

A variety of archaeal lineages have been identified using culture-independent molecular phylogenetic surveys of microbial habitats occurring in deep-sea hydrothermal environments such as chimney structures, sediments, vent emissions, and chemosynthetic macrofauna. With the exception of a few taxa, most of these archaea have not yet been cultivated, and their physiological and metabolic traits remain unclear. In this study, phylogenetic diversity and distribution profiles of the archaeal genes encoding small subunit (SSU) rRNA, methyl coenzyme A (CoA) reductase subunit A, and the ammonia monooxygenase large subunit were characterized in hydrothermally influenced sediments at the Yonaguni Knoll IV hydrothermal field in the Southern Okinawa Trough. Sediment cores were collected at distances of 0.5, 2, or 5 m from a vent emission (90°C). A moderate temperature gradient extends both horizontally and vertically (5 to 69°C), indicating the existence of moderate mixing between the hydrothermal fluid and the ambient sediment pore water. The mixing of reductive hot hydrothermal fluid and cold ambient sediment pore water establishes a wide spectrum of physical and chemical conditions in the microbial habitats that were investigated. Under these different physico-chemical conditions, variability in archaeal phylotype composition was observed. The relationship between the physical and chemical parameters and the archaeal phylotype composition provides important insight into the ecophysiological requirements of uncultivated archaeal lineages in deep-sea hydrothermal vent environments, giving clues for approximating culture conditions to be used in future culturing efforts.

Original languageEnglish
Pages (from-to)1198-1211
Number of pages14
JournalApplied and environmental microbiology
Volume76
Issue number4
DOIs
Publication statusPublished - Feb 1 2010

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Hydrothermal Vents
Ryukyu Archipelago
Ecosystem
Archaeal Genes
trough
Hot Temperature
heat
sediments
Water
Archaea
Coenzyme A
hydrothermal fluid
Oceans and Seas
sediment
deep sea
porewater
Oxidoreductases
phylogenetics
Temperature
hydrothermal vent

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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Archaeal diversity and distribution along thermal and geochemical gradients in hydrothermal sediments at the yonaguni knoll IV hydrothermal field in the Southern Okinawa Trough. / Nunoura, Takuro; Oida, Hanako; Nakaseama, Miwako; Kosaka, Ayako; Ohkubo, Satoru B.; Kikuchi, Toru; Kazama, Hiromi; Hosoi-Tanabe, Shoko; Nakamura, Ko Ichi; Kinoshita, Masataka; Hirayama, Hisako; Inagaki, Fumio; Tsunogai, Urumu; Ishibashi, Jun Ichiro; Takai, Ken.

In: Applied and environmental microbiology, Vol. 76, No. 4, 01.02.2010, p. 1198-1211.

Research output: Contribution to journalArticle

Nunoura, T, Oida, H, Nakaseama, M, Kosaka, A, Ohkubo, SB, Kikuchi, T, Kazama, H, Hosoi-Tanabe, S, Nakamura, KI, Kinoshita, M, Hirayama, H, Inagaki, F, Tsunogai, U, Ishibashi, JI & Takai, K 2010, 'Archaeal diversity and distribution along thermal and geochemical gradients in hydrothermal sediments at the yonaguni knoll IV hydrothermal field in the Southern Okinawa Trough', Applied and environmental microbiology, vol. 76, no. 4, pp. 1198-1211. https://doi.org/10.1128/AEM.00924-09
Nunoura, Takuro ; Oida, Hanako ; Nakaseama, Miwako ; Kosaka, Ayako ; Ohkubo, Satoru B. ; Kikuchi, Toru ; Kazama, Hiromi ; Hosoi-Tanabe, Shoko ; Nakamura, Ko Ichi ; Kinoshita, Masataka ; Hirayama, Hisako ; Inagaki, Fumio ; Tsunogai, Urumu ; Ishibashi, Jun Ichiro ; Takai, Ken. / Archaeal diversity and distribution along thermal and geochemical gradients in hydrothermal sediments at the yonaguni knoll IV hydrothermal field in the Southern Okinawa Trough. In: Applied and environmental microbiology. 2010 ; Vol. 76, No. 4. pp. 1198-1211.
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