Architecture of the DNA polymerase B-proliferating cell nuclear antigen (PCNA)-DNA ternary complex

Kouta Mayanagi, Shinichi Kiyonari, Hirokazu Nishida, Mihoko Saito, Daisuke Kohda, Yoshizumi Ishino, Tsuyoshi Shirai, Kosuke Morikawa

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

DNA replication in archaea and eukaryotes is executed by family B DNA polymerases, which exhibit full activity when complexed with the DNA clamp, proliferating cell nuclear antigen (PCNA). This replication enzyme consists of the polymerase and exonuclease moieties responsible for DNA synthesis and editing (proofreading), respectively. Because of the editing activity, this enzyme ensures the high fidelity of DNA replication. However, it remains unclear how the PCNA-complexed enzyme temporally switches between the polymerizing and editing modes. Here, we present the three-dimensional structure of the Pyrococcus furiosus DNA polymerase B-PCNA-DNA ternary complex, which is the core component of the replisome, determined by single particle electron microscopy of negatively stained samples. This structural view, representing the complex in the editing mode, revealed the whole domain configuration of the trimeric PCNA ring and the DNA polymerase, including protein-protein and protein-DNA contacts. Notably, besides the authentic DNA polymerase-PCNA interaction through a PCNA-interacting protein (PIP) box, a novel contact was found between DNA polymerase and the PCNA subunit adjacent to that with the PIP contact. This contact appears to be responsible for the configuration of the complex specific for the editing mode. The DNA was located almost at the center of PCNA and exhibited a substantial and particular tilt angle against the PCNA ring plane. The obtained molecular architecture of the complex, including the new contact found in this work, provides clearer insights into the switching mechanism between the two distinct modes, thus highlighting the functional significance of PCNA in the replication process.

Original languageEnglish
Pages (from-to)1845-1849
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number5
DOIs
Publication statusPublished - Feb 1 2011

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Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
DNA
Proteins
DNA Replication
Enzymes
Pyrococcus furiosus
Exonucleases
Archaea
Eukaryota
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • General

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Architecture of the DNA polymerase B-proliferating cell nuclear antigen (PCNA)-DNA ternary complex. / Mayanagi, Kouta; Kiyonari, Shinichi; Nishida, Hirokazu; Saito, Mihoko; Kohda, Daisuke; Ishino, Yoshizumi; Shirai, Tsuyoshi; Morikawa, Kosuke.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 5, 01.02.2011, p. 1845-1849.

Research output: Contribution to journalArticle

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