TY - JOUR
T1 - Are free radical reactions increased in the diabetic eye?
AU - Yamato, Mayumi
AU - Matsumoto, Shingo
AU - Ura, Katsuaki
AU - Yamada, Ken Ichi
AU - Naganuma, Tatsuya
AU - Inoguchi, Toyoshi
AU - Watanabe, Toshiaki
AU - Utsumi, Hideo
PY - 2007/3
Y1 - 2007/3
N2 - Reactive oxygen species (ROS) are thought to play a significant role in the development of diabetic retinopathy; however, no direct evidence supports ROS generation in vivo. This study used in vivo electron spin resonance (ESR) spectroscopy with a surface resonator to detect local free radical reactions. The ESR signal decay of carbamoyl-PROXYL was enhanced in the eyes of streptozotocin (STZ)-induced diabetic mice. This enhanced signal decay was suppressed by the administration of SOD or the pretreatment with aminoguanidine. We demonstrate, for the first time, specific free radical reactions in the eyes of mice with STZ-induced diabetes.
AB - Reactive oxygen species (ROS) are thought to play a significant role in the development of diabetic retinopathy; however, no direct evidence supports ROS generation in vivo. This study used in vivo electron spin resonance (ESR) spectroscopy with a surface resonator to detect local free radical reactions. The ESR signal decay of carbamoyl-PROXYL was enhanced in the eyes of streptozotocin (STZ)-induced diabetic mice. This enhanced signal decay was suppressed by the administration of SOD or the pretreatment with aminoguanidine. We demonstrate, for the first time, specific free radical reactions in the eyes of mice with STZ-induced diabetes.
UR - http://www.scopus.com/inward/record.url?scp=33846436066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846436066&partnerID=8YFLogxK
U2 - 10.1089/ars.2006.1502
DO - 10.1089/ars.2006.1502
M3 - Review article
C2 - 17184178
AN - SCOPUS:33846436066
SN - 1523-0864
VL - 9
SP - 367
EP - 373
JO - Antioxidants and Redox Signaling
JF - Antioxidants and Redox Signaling
IS - 3
ER -
