Arginine residues in the extension peptide are required for cleavage of a precursor by mitochondrial processing peptidase. Demonstration using synthetic peptide as a substrate

T. Niidome, S. Kitada, K. Shimokata, T. Ogishima, A. Ito

Research output: Contribution to journalArticle

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Abstract

Mitochondrial processing peptidase (MPP) specifically recognizes a large variety of mitochondrial precursor proteins and correctly cleaves off the extension peptides. To determine the structure common to all the extension peptides that is required for specific recognition by MPP, we synthesized various oligopeptides of different chain lengths and amino acid sequences, based on the amino acid sequence of the extension peptide of pre-malate dehydrogenase, and determined kinetic parameters of the cleavage reactions. The minimal length of peptides for effective cleavage was 16 amino acid residues consisting of 11 and 5 residues from the cleavage site to the amino- and carboxyl-terminal sides, respectively. Two sets of basic amino acids in the peptide, the distal arginine residue at position -10 and the proximal ones at positions -3 and -2 relative to the cleavage site, were necessary for effective hydrolysis. Of these two, the residue at position -2 was more important for effective cleavage than the one at position -3 and could not be replaced by a lysine residue. The replacement of the distal arginine by lysine had no effect on the cleavage. Our study demonstrates that use of peptides with the proper length is essential for performing kinetic analyses on the cleavage reaction by MPP and that an arginine residue at position -2 to the cleavage site is necessary for the recognition and cleavage of the extension peptide.

Original languageEnglish
Pages (from-to)24719-24722
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number40
Publication statusPublished - Jan 1 1994

Fingerprint

Arginine
Demonstrations
Peptides
Substrates
Amino Acids
Lysine
Amino Acid Sequence
Basic Amino Acids
Malate Dehydrogenase
Oligopeptides
Protein Precursors
Mitochondrial Proteins
mitochondrial processing peptidase
Chain length
Kinetic parameters
Hydrolysis
Kinetics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Arginine residues in the extension peptide are required for cleavage of a precursor by mitochondrial processing peptidase. Demonstration using synthetic peptide as a substrate. / Niidome, T.; Kitada, S.; Shimokata, K.; Ogishima, T.; Ito, A.

In: Journal of Biological Chemistry, Vol. 269, No. 40, 01.01.1994, p. 24719-24722.

Research output: Contribution to journalArticle

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