Armet is an effector protein mediating aphid-plant interactions

Wei Wang, Huaien Dai, Yi Zhang, Raman Chandrasekar, Lan Luo, Yasuaki Hiromasa, Changzhong Sheng, Gongxin Peng, Shaoliang Chen, John M. Tomich, John Reese, Owain Edwards, Le Kang, Gerald Reeck, Feng Cui

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Aphid saliva is predicted to contain proteins that modulate plant defenses and facilitate feeding. Armet is a well-characterized bifunctional protein in mammalian systems. Here we report a new role of Armet, namely as an effector protein in the pea aphid, Acyrthosiphon pisum. Pea aphid Armet's physical and chemical properties and its intracellular role are comparable to those reported for mammalian Armets. Uniquely, we detected Armet in aphid watery saliva and in the phloem sap of fava beans fed on by aphids. Armet's transcript level is several times higher in the salivary gland when aphids feed on bean plants than when they feed on an artificial diet. Knockdown of the Armet transcript by RNA interference disturbs aphid feeding behavior on fava beans measured by the electrical penetration graph technique and leads to a shortened life span. Inoculation of pea aphid Armet protein into tobacco leaves induced a transcriptional response that included pathogenresponsive genes. The data suggest that Armet is an effector protein mediating aphid-plant interactions.

Original languageEnglish
Pages (from-to)2032-2045
Number of pages14
JournalFASEB Journal
Volume29
Issue number5
DOIs
Publication statusPublished - May 1 2015
Externally publishedYes

Fingerprint

Aphids
Proteins
Peas
Plant Proteins
Tobacco
Vicia faba
Nutrition
Chemical properties
Saliva
Physical properties
Genes
RNA
Phloem
Feeding Behavior
RNA Interference
Salivary Glands
Diet

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Wang, W., Dai, H., Zhang, Y., Chandrasekar, R., Luo, L., Hiromasa, Y., ... Cui, F. (2015). Armet is an effector protein mediating aphid-plant interactions. FASEB Journal, 29(5), 2032-2045. https://doi.org/10.1096/fj.14-266023

Armet is an effector protein mediating aphid-plant interactions. / Wang, Wei; Dai, Huaien; Zhang, Yi; Chandrasekar, Raman; Luo, Lan; Hiromasa, Yasuaki; Sheng, Changzhong; Peng, Gongxin; Chen, Shaoliang; Tomich, John M.; Reese, John; Edwards, Owain; Kang, Le; Reeck, Gerald; Cui, Feng.

In: FASEB Journal, Vol. 29, No. 5, 01.05.2015, p. 2032-2045.

Research output: Contribution to journalArticle

Wang, W, Dai, H, Zhang, Y, Chandrasekar, R, Luo, L, Hiromasa, Y, Sheng, C, Peng, G, Chen, S, Tomich, JM, Reese, J, Edwards, O, Kang, L, Reeck, G & Cui, F 2015, 'Armet is an effector protein mediating aphid-plant interactions', FASEB Journal, vol. 29, no. 5, pp. 2032-2045. https://doi.org/10.1096/fj.14-266023
Wang W, Dai H, Zhang Y, Chandrasekar R, Luo L, Hiromasa Y et al. Armet is an effector protein mediating aphid-plant interactions. FASEB Journal. 2015 May 1;29(5):2032-2045. https://doi.org/10.1096/fj.14-266023
Wang, Wei ; Dai, Huaien ; Zhang, Yi ; Chandrasekar, Raman ; Luo, Lan ; Hiromasa, Yasuaki ; Sheng, Changzhong ; Peng, Gongxin ; Chen, Shaoliang ; Tomich, John M. ; Reese, John ; Edwards, Owain ; Kang, Le ; Reeck, Gerald ; Cui, Feng. / Armet is an effector protein mediating aphid-plant interactions. In: FASEB Journal. 2015 ; Vol. 29, No. 5. pp. 2032-2045.
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