Array-based analysis on tobacco plastid transcripts: Preparation of a genomic microarray containing all genes and all intergenic regions

Takahiro Nakamura, Yumiko Furuhashi, Keiko Hasegawa, Hiroshi Hashimoto, Kazufumi Watanabe, Junichi Obokata, Mamoru Sugita, Masahiro Sugiura

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The plastid genome of higher plants includes about 120 genes. We adopted genomic array technologies to the tobacco plastid genome. A microarray was constructed, consisting of 220 DNA fragments that cover the whole genome sequence. Each DNA fragment corresponds to a single known gene or an intergenic region. We evaluated reliability of this microarray by comparing the plastid RNA level in light- or dark-grown tobacco seedlings. The transcripts encoding photosynthetic subunits increased significantly in light-grown tissues as expected. Furthermore, we found unexpected signals in several intergenic regions, suggesting the existence of novel transcripts in tobacco plastids.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalPlant and Cell Physiology
Volume44
Issue number8
DOIs
Publication statusPublished - Aug 1 2003
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this