Array-based analysis on tobacco plastid transcripts

Preparation of a genomic microarray containing all genes and all intergenic regions

Takahiro Nakamura, Yumiko Furuhashi, Keiko Hasegawa, Hiroshi Hashimoto, Kazufumi Watanabe, Junichi Obokata, Mamoru Sugita, Masahiro Sugiura

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The plastid genome of higher plants includes about 120 genes. We adopted genomic array technologies to the tobacco plastid genome. A microarray was constructed, consisting of 220 DNA fragments that cover the whole genome sequence. Each DNA fragment corresponds to a single known gene or an intergenic region. We evaluated reliability of this microarray by comparing the plastid RNA level in light- or dark-grown tobacco seedlings. The transcripts encoding photosynthetic subunits increased significantly in light-grown tissues as expected. Furthermore, we found unexpected signals in several intergenic regions, suggesting the existence of novel transcripts in tobacco plastids.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalPlant and Cell Physiology
Volume44
Issue number8
DOIs
Publication statusPublished - Aug 1 2003

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Intergenic DNA
Plastids
Plastid Genomes
intergenic DNA
plastids
Tobacco
tobacco
genomics
Genes
Light
genes
DNA
Seedlings
Genome
RNA
Technology
genome
seedlings
plastid genome

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Array-based analysis on tobacco plastid transcripts : Preparation of a genomic microarray containing all genes and all intergenic regions. / Nakamura, Takahiro; Furuhashi, Yumiko; Hasegawa, Keiko; Hashimoto, Hiroshi; Watanabe, Kazufumi; Obokata, Junichi; Sugita, Mamoru; Sugiura, Masahiro.

In: Plant and Cell Physiology, Vol. 44, No. 8, 01.08.2003, p. 861-867.

Research output: Contribution to journalArticle

Nakamura, Takahiro ; Furuhashi, Yumiko ; Hasegawa, Keiko ; Hashimoto, Hiroshi ; Watanabe, Kazufumi ; Obokata, Junichi ; Sugita, Mamoru ; Sugiura, Masahiro. / Array-based analysis on tobacco plastid transcripts : Preparation of a genomic microarray containing all genes and all intergenic regions. In: Plant and Cell Physiology. 2003 ; Vol. 44, No. 8. pp. 861-867.
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