Arsenate and microbial dynamics in different phosphorus regimes of the subtropical Pacific Ocean

Fuminori Hashihama, Shuhei Suwa, Jota Kanda, Makoto Ehama, Ryousuke Sakuraba, Shinko Kinouchi, Mitsuhide Sato, Tamaha Yamaguchi, Hiroaki Saito, Yoshitoshi Ogura, Tetsuya Hayashi, Hiroshi Mori, Ken Kurokawa, Shotaro Suzuki, Koji Hamasaki

Research output: Contribution to journalArticle

Abstract

Biologically toxic arsenate is physicochemically similar to biologically essential phosphate. Because arsenate and phosphate are indiscriminately incorporated by microbes, their ambient concentration ratios can be an important factor controlling microbial growth and metabolism. This study investigated the spatial distributions of arsenate and phosphate and the associated biogeochemical dynamics in the subtropical North and South Pacific Ocean. Vertical arsenate and phosphate profiles (≤ 150 m) in most of the study areas showed a nutrient-type distribution where the concentrations increased below the euphotic zone. The arsenate and phosphate concentrations in the surface waters ranged from the detection limits (5 nM and 4 nM, respectively) to approximately 40 nM and 400 nM, respectively. The surface arsenate:phosphate ratios were typically lower than 1, but those in the western subtropical North Pacific (WSNP) were frequently higher than 1 due to phosphate depletion. In the WSNP surface waters, Prochlorococcus and Pelagibacter arsenic detoxification and phosphorus acquisition genes were abundant. Results of the onboard bioassays involving the addition of arsenate or phosphate to the surface water indicated that microbes throughout the study areas possessed arsenate resistance and those in the WSNP during summer were under serious phosphate limitation. Although phosphate limitation likely accelerates the relative cellular accumulation of toxic arsenate, the lowest particulate As:P ratios were observed in the summer WSNP, concurrent with the lowest dissolved organic P (DOP) concentrations and the highest alkaline phosphatase activities. These results imply that active As excretion and/or DOP utilization could alleviate As accumulation while maintaining the cellular P quota.

Original languageEnglish
Article number102115
JournalProgress in Oceanography
Volume176
DOIs
Publication statusPublished - Sep 2019

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arsenates
arsenate
Pacific Ocean
phosphate
phosphates
phosphorus
ocean
surface water
microorganisms
Prochlorococcus
summer
euphotic zone
detoxification
arsenic
phosphatase
excretion
alkaline phosphatase
microbial growth
bioassay
particulates

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Geology

Cite this

Hashihama, F., Suwa, S., Kanda, J., Ehama, M., Sakuraba, R., Kinouchi, S., ... Hamasaki, K. (2019). Arsenate and microbial dynamics in different phosphorus regimes of the subtropical Pacific Ocean. Progress in Oceanography, 176, [102115]. https://doi.org/10.1016/j.pocean.2019.05.007

Arsenate and microbial dynamics in different phosphorus regimes of the subtropical Pacific Ocean. / Hashihama, Fuminori; Suwa, Shuhei; Kanda, Jota; Ehama, Makoto; Sakuraba, Ryousuke; Kinouchi, Shinko; Sato, Mitsuhide; Yamaguchi, Tamaha; Saito, Hiroaki; Ogura, Yoshitoshi; Hayashi, Tetsuya; Mori, Hiroshi; Kurokawa, Ken; Suzuki, Shotaro; Hamasaki, Koji.

In: Progress in Oceanography, Vol. 176, 102115, 09.2019.

Research output: Contribution to journalArticle

Hashihama, F, Suwa, S, Kanda, J, Ehama, M, Sakuraba, R, Kinouchi, S, Sato, M, Yamaguchi, T, Saito, H, Ogura, Y, Hayashi, T, Mori, H, Kurokawa, K, Suzuki, S & Hamasaki, K 2019, 'Arsenate and microbial dynamics in different phosphorus regimes of the subtropical Pacific Ocean', Progress in Oceanography, vol. 176, 102115. https://doi.org/10.1016/j.pocean.2019.05.007
Hashihama, Fuminori ; Suwa, Shuhei ; Kanda, Jota ; Ehama, Makoto ; Sakuraba, Ryousuke ; Kinouchi, Shinko ; Sato, Mitsuhide ; Yamaguchi, Tamaha ; Saito, Hiroaki ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Mori, Hiroshi ; Kurokawa, Ken ; Suzuki, Shotaro ; Hamasaki, Koji. / Arsenate and microbial dynamics in different phosphorus regimes of the subtropical Pacific Ocean. In: Progress in Oceanography. 2019 ; Vol. 176.
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