Artemisinin production by shoot regeneration of Artemisia annua L. using thidiazuron

Wanwimon Lualon, Wanchai De-Eknamkul, Hiroyuki Tanaka, Yukihiro Shoyama, Waraporn Putalun

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem expiants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/explant) after 2 weeks of culture. Healthy regenerated shoots were elongated and rooted in MS medium without hormones. The artemisinin content in plants regenerated from stem expiants using 0.1 mg/l TDZ was (3.36 ± 0.36) μg/mg dry weight and two-fold higher than that of in vitro grown plants of the same age [(1.73 ± 0.23) μ/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem expiant and makes it possible to develop a clonal propagation of A. annua.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Issue number1-2
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)


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