TY - JOUR
T1 - Artemisinin production by shoot regeneration of Artemisia annua L. using thidiazuron
AU - Lualon, Wanwimon
AU - De-Eknamkul, Wanchai
AU - Tanaka, Hiroyuki
AU - Shoyama, Yukihiro
AU - Putalun, Waraporn
PY - 2008/1/1
Y1 - 2008/1/1
N2 - An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem expiants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/explant) after 2 weeks of culture. Healthy regenerated shoots were elongated and rooted in MS medium without hormones. The artemisinin content in plants regenerated from stem expiants using 0.1 mg/l TDZ was (3.36 ± 0.36) μg/mg dry weight and two-fold higher than that of in vitro grown plants of the same age [(1.73 ± 0.23) μ/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem expiant and makes it possible to develop a clonal propagation of A. annua.
AB - An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem expiants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/explant) after 2 weeks of culture. Healthy regenerated shoots were elongated and rooted in MS medium without hormones. The artemisinin content in plants regenerated from stem expiants using 0.1 mg/l TDZ was (3.36 ± 0.36) μg/mg dry weight and two-fold higher than that of in vitro grown plants of the same age [(1.73 ± 0.23) μ/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem expiant and makes it possible to develop a clonal propagation of A. annua.
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U2 - 10.1515/znc-2008-1-218
DO - 10.1515/znc-2008-1-218
M3 - Article
C2 - 18386496
AN - SCOPUS:40549117894
VL - 63
SP - 96
EP - 100
JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
SN - 0939-5075
IS - 1-2
ER -