Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways

Yoshio Kano, Noboru Horie, Shima Doi, Fumika Aramaki, Hidefumi Maeda, Fukumi Hiragami, Kenji Kawamura, Hirotoshi Motoda, Yoshihisa Koike, Junichi Akiyama, Sueo Eguchi, Ken Hashimoto

Research output: Contribution to journalArticle

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Abstract

We investigated whether artepillin C, a major component of Brazilian propolis, acts as a neurotrophic-like factor in rat PC12m3 cells, in which nerve growth factor (NGF)-induced neurite outgrowth is impaired. When cultures of PC12m3 cells were treated with artepillin C at a concentration of 20 μM, the frequency of neurite outgrowth induced by artepillin C was approximately 7-fold greater than that induced by NGF alone. Artepillin C induced-neurite outgrowth of PC12m3 cells was inhibited by the ERK inhibitor U0126 and by the p38 MAPK inhibitor SB203580. Although artepillin C-induced p38 MAPK activity was detected in PC12m3 cells, phosphorylation of ERK induced by artepillin C was not observed. On the other hand, artepillin C caused rapid activation of ERK and the time course of the activation was similar to that induced by NGF treatment in PC12 parental cells. However, NGF-induced neurite outgrowth was inhibited by artepillin C treatment. Interestingly, inhibition of ERK by U0126 completely prevented artepillin C-induced p38 MAPK phosphorylation of PC12m3 cells. These findings suggest that artepillin C-induced activation of p38 MAPK through the ERK signaling pathway is responsible for the neurite outgrowth of PC12m3 cells.

Original languageEnglish
Pages (from-to)1795-1803
Number of pages9
JournalNeurochemical Research
Volume33
Issue number9
DOIs
Publication statusPublished - Sep 1 2008

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Propolis
p38 Mitogen-Activated Protein Kinases
Nerve Growth Factor
Phosphorylation
Chemical activation
artepillin C
Neuronal Outgrowth
MAP Kinase Signaling System
PC12 Cells
Nerve Growth Factors
Rats
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways. / Kano, Yoshio; Horie, Noboru; Doi, Shima; Aramaki, Fumika; Maeda, Hidefumi; Hiragami, Fukumi; Kawamura, Kenji; Motoda, Hirotoshi; Koike, Yoshihisa; Akiyama, Junichi; Eguchi, Sueo; Hashimoto, Ken.

In: Neurochemical Research, Vol. 33, No. 9, 01.09.2008, p. 1795-1803.

Research output: Contribution to journalArticle

Kano, Y, Horie, N, Doi, S, Aramaki, F, Maeda, H, Hiragami, F, Kawamura, K, Motoda, H, Koike, Y, Akiyama, J, Eguchi, S & Hashimoto, K 2008, 'Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways', Neurochemical Research, vol. 33, no. 9, pp. 1795-1803. https://doi.org/10.1007/s11064-008-9633-9
Kano, Yoshio ; Horie, Noboru ; Doi, Shima ; Aramaki, Fumika ; Maeda, Hidefumi ; Hiragami, Fukumi ; Kawamura, Kenji ; Motoda, Hirotoshi ; Koike, Yoshihisa ; Akiyama, Junichi ; Eguchi, Sueo ; Hashimoto, Ken. / Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways. In: Neurochemical Research. 2008 ; Vol. 33, No. 9. pp. 1795-1803.
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