A novel artificial biocatalyst that catalyzes a hydrolysis reaction was prepared by a surface molecular imprinting technique. The properties of the artificial biocatalysts were investigated in the hydrolysis reaction of an amino acid derivative in a biphasic system. The enzyme-mimic biocatalyst showed a high activity and maintained high stability for a long time. We demonstrate that the surface molecular imprinting technique is effective for designing novel artificial enzyme materials.
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