Artificial golgi apparatus

Globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis

Takahiko Matsushita, Izuru Nagashima, Masataka Fumoto, Takashi Ohta, Kuriko Yamada, Hiroki Shimizu, Hiroshi Hinou, Kentaro Naruchi, Takaomi Ito, Hirosato Kondo, Shin Ichiro Nishimura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Despite the growing importance of synthetic glycans as tools for biological studies and drug discovery, a lack of common methods for the routine synthesis remains a major obstacle. We have developed a new method for automated glycan synthesis that employs the enzymatic approach and a dendrimer as an ideal support within the chemical process. Recovery tests using a hollow fiber ultrafiltration module have revealed that monodisperse G6 (MW = 58 kDa) and G7 (MW = 116 kDa) poly(amidoamine) dendrimers exhibit a similar profile to BSA (MW = 66 kDa). Characteristics of the globular protein-like G7 dendrimer with high solubility and low viscosity in water greatly enhanced throughput and efficiency in automated synthesis while random polyacrylamide-based supports entail significant loss during the repetitive reaction/separation step. The present protocol allowed for the fully automated enzymatic synthesis of sialyl Lewis X tetrasaccharide derivatives over a period of 4 days in 16% overall yield from a simple N-acetyl-d-glucosamine linked to an aminooxy-functionalized G7 dendrimer.

Original languageEnglish
Pages (from-to)16651-16656
Number of pages6
JournalJournal of the American Chemical Society
Volume132
Issue number46
DOIs
Publication statusPublished - Nov 24 2010
Externally publishedYes

Fingerprint

Dendrimers
Golgi Apparatus
Polysaccharides
Proteins
Chemical Phenomena
Glucosamine
Ultrafiltration
Drug Discovery
Polyacrylates
Viscosity
Solubility
Throughput
Derivatives
Recovery
Water
Fibers

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Matsushita, T., Nagashima, I., Fumoto, M., Ohta, T., Yamada, K., Shimizu, H., ... Nishimura, S. I. (2010). Artificial golgi apparatus: Globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis. Journal of the American Chemical Society, 132(46), 16651-16656. https://doi.org/10.1021/ja106955j

Artificial golgi apparatus : Globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis. / Matsushita, Takahiko; Nagashima, Izuru; Fumoto, Masataka; Ohta, Takashi; Yamada, Kuriko; Shimizu, Hiroki; Hinou, Hiroshi; Naruchi, Kentaro; Ito, Takaomi; Kondo, Hirosato; Nishimura, Shin Ichiro.

In: Journal of the American Chemical Society, Vol. 132, No. 46, 24.11.2010, p. 16651-16656.

Research output: Contribution to journalArticle

Matsushita, T, Nagashima, I, Fumoto, M, Ohta, T, Yamada, K, Shimizu, H, Hinou, H, Naruchi, K, Ito, T, Kondo, H & Nishimura, SI 2010, 'Artificial golgi apparatus: Globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis', Journal of the American Chemical Society, vol. 132, no. 46, pp. 16651-16656. https://doi.org/10.1021/ja106955j
Matsushita, Takahiko ; Nagashima, Izuru ; Fumoto, Masataka ; Ohta, Takashi ; Yamada, Kuriko ; Shimizu, Hiroki ; Hinou, Hiroshi ; Naruchi, Kentaro ; Ito, Takaomi ; Kondo, Hirosato ; Nishimura, Shin Ichiro. / Artificial golgi apparatus : Globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 46. pp. 16651-16656.
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