Artificial self-sufficient P450 in reversed micelles

Hidehiko Hirakawa, Noriho Kamiya, Yutaka Kawarabayasi, Teruyuki Nagamune

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cytochrome P450s are heme-containing monooxygenases that require electron transfer proteins for their catalytic activities. They prefer hydrophobic compounds as substrates and it is, therefore, desirable to perform their reactions in non-aqueous media. Reversed micelles can stably encapsulate proteins in nano-scaled water pools in organic solvents. However, in the reversed micellar system, when multiple proteins are involved in a reaction they can be separated into different micelles and it is then difficult to transfer electrons between proteins. We show here that an artificial self-sufficient cytochrome P450, which is an enzymatically crosslinked fusion protein composed of P450 and electron transfer proteins, showed micelle-size dependent catalytic activity in a reversed micellar system. Furthermore, the presence of thermostable alcohol dehydrogenase promoted the P450-catalyzed reaction due to cofactor regeneration.

Original languageEnglish
Pages (from-to)2935-2948
Number of pages14
JournalMolecules
Volume15
Issue number5
DOIs
Publication statusPublished - May 1 2010

Fingerprint

Micelles
micelles
proteins
electron transfer
Proteins
cytochromes
Electrons
catalytic activity
Catalyst activity
dehydrogenases
Alcohol Dehydrogenase
Cytochromes
Mixed Function Oxygenases
regeneration
Heme
Organic solvents
Cytochrome P-450 Enzyme System
Regeneration
alcohols
Fusion reactions

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Medicine(all)

Cite this

Hirakawa, H., Kamiya, N., Kawarabayasi, Y., & Nagamune, T. (2010). Artificial self-sufficient P450 in reversed micelles. Molecules, 15(5), 2935-2948. https://doi.org/10.3390/molecules15052935

Artificial self-sufficient P450 in reversed micelles. / Hirakawa, Hidehiko; Kamiya, Noriho; Kawarabayasi, Yutaka; Nagamune, Teruyuki.

In: Molecules, Vol. 15, No. 5, 01.05.2010, p. 2935-2948.

Research output: Contribution to journalArticle

Hirakawa, H, Kamiya, N, Kawarabayasi, Y & Nagamune, T 2010, 'Artificial self-sufficient P450 in reversed micelles', Molecules, vol. 15, no. 5, pp. 2935-2948. https://doi.org/10.3390/molecules15052935
Hirakawa, Hidehiko ; Kamiya, Noriho ; Kawarabayasi, Yutaka ; Nagamune, Teruyuki. / Artificial self-sufficient P450 in reversed micelles. In: Molecules. 2010 ; Vol. 15, No. 5. pp. 2935-2948.
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