Ascidian sperm activating and attracting factor: Importance of sulfate groups for the activities and implication of its putative receptor

Manabu Yoshida, Kogiku Shiba, Kaoru Yoshida, Hiroshi Tsuchikawa, Ouichirou Ootou, Toru Oishi, Michio Murata

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Ciona intestinalis was identified as the sulfated polyhydroxysterol,3α,4β,7α,26-tetrahydroxy-5α-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.

Original languageEnglish
Pages (from-to)3429-3433
Number of pages5
JournalFEBS Letters
Volume582
Issue number23-24
DOIs
Publication statusPublished - Oct 15 2008
Externally publishedYes

Fingerprint

Urochordata
Sulfates
Spermatozoa
Hydrolysis
Cholestanes
Functional analysis
Functional groups
Derivatives
Ciona intestinalis
Isomerism
Eggs

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ascidian sperm activating and attracting factor : Importance of sulfate groups for the activities and implication of its putative receptor. / Yoshida, Manabu; Shiba, Kogiku; Yoshida, Kaoru; Tsuchikawa, Hiroshi; Ootou, Ouichirou; Oishi, Toru; Murata, Michio.

In: FEBS Letters, Vol. 582, No. 23-24, 15.10.2008, p. 3429-3433.

Research output: Contribution to journalArticle

Yoshida, Manabu ; Shiba, Kogiku ; Yoshida, Kaoru ; Tsuchikawa, Hiroshi ; Ootou, Ouichirou ; Oishi, Toru ; Murata, Michio. / Ascidian sperm activating and attracting factor : Importance of sulfate groups for the activities and implication of its putative receptor. In: FEBS Letters. 2008 ; Vol. 582, No. 23-24. pp. 3429-3433.
@article{5dbf04f5796e4cc0adb64e1016d507d0,
title = "Ascidian sperm activating and attracting factor: Importance of sulfate groups for the activities and implication of its putative receptor",
abstract = "The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Ciona intestinalis was identified as the sulfated polyhydroxysterol,3α,4β,7α,26-tetrahydroxy-5α-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.",
author = "Manabu Yoshida and Kogiku Shiba and Kaoru Yoshida and Hiroshi Tsuchikawa and Ouichirou Ootou and Toru Oishi and Michio Murata",
year = "2008",
month = "10",
day = "15",
doi = "10.1016/j.febslet.2008.09.006",
language = "English",
volume = "582",
pages = "3429--3433",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "23-24",

}

TY - JOUR

T1 - Ascidian sperm activating and attracting factor

T2 - Importance of sulfate groups for the activities and implication of its putative receptor

AU - Yoshida, Manabu

AU - Shiba, Kogiku

AU - Yoshida, Kaoru

AU - Tsuchikawa, Hiroshi

AU - Ootou, Ouichirou

AU - Oishi, Toru

AU - Murata, Michio

PY - 2008/10/15

Y1 - 2008/10/15

N2 - The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Ciona intestinalis was identified as the sulfated polyhydroxysterol,3α,4β,7α,26-tetrahydroxy-5α-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.

AB - The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Ciona intestinalis was identified as the sulfated polyhydroxysterol,3α,4β,7α,26-tetrahydroxy-5α-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.

UR - http://www.scopus.com/inward/record.url?scp=53049092876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53049092876&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.09.006

DO - 10.1016/j.febslet.2008.09.006

M3 - Article

C2 - 18789324

AN - SCOPUS:53049092876

VL - 582

SP - 3429

EP - 3433

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 23-24

ER -