A high-performance liquid chromatography method for the assay of Escherichia coli photolyase activity was developed. When cis-syn cyclobutane pyrimidine dimer was used as substrate, the Michaelis constant (Km) value for the photolyase activity was 100nM. The linear range of the calibration curve of the photolyase activity was 0.026-6.64μU/assay tube. The correlation coefficient for this linearity was 0.998. The limit of detection (S/N=3) was 26nU/assay tube. The photolyase activity was increased 1.6-fold in the presence of 5,10-methenyltetrahydrofolic acid in the enzyme reaction mixture.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology